Tetanus Toxin Antibody - BSA Free

Novus Biologicals | Catalog # NB100-63239

Novus Biologicals
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Key Product Details

Species Reactivity

Bacteria

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Tetanus Toxin Primary Antibodies »

Product Specifications

Immunogen

Tetanus Exotoxin

Localization

Secreted

Specificity

NB100-63239 detects the Tetanus Toxin which is produced by the soil micro-organism Clostridium tetani. C. tetaniinfection results in its systemic growth and secretion of tetanus, which causes muscle spasms by inhibiting neurotransmitter release. The antibody also reacts with the C-fragment of tetanus toxin.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Tetanus Toxin Antibody - BSA Free

Western Blot: Tetanus Toxin AntibodyBSA Free [NB100-63239]

Western Blot: Tetanus Toxin AntibodyBSA Free [NB100-63239]

Western Blot: Tetanus Toxin Antibody [NB100-63239] - 1 ug of tetanus toxin (Sigma T3194-25UG) per well.

Applications for Tetanus Toxin Antibody - BSA Free

Application
Recommended Usage

ELISA

1 ug/ml

Western Blot

1:100-1:500
Application Notes
By WB, it detects the complete toxin (faint 150 kDa band), the heavy chain (main 100 kDa band) and the light chain (faint 50 kDa band).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

5.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tetanus Toxin

Tetanus Toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2. The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.

Alternate Names

Tentoxylysin

Gene Symbol

TETX

Additional Tetanus Toxin Products

Product Documents for Tetanus Toxin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Tetanus Toxin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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