Themis Antibody

Novus Biologicals | Catalog # NBP2-27492

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Themis Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EERTINLPKSLKSH corresponding to C-Terminus according to NP_848781.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat Themis Antibody (NBP2-27492) is a polyclonal antibody validated for use in WB and ELISA. Anti-Themis Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Themis Antibody

Western Blot: Themis Antibody [NBP2-27492]

Western Blot: Themis Antibody [NBP2-27492]

Western Blot: THEMIS Antibody [NBP2-27492] - (0.3 ug/ml) Analysis of Mouse Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Themis Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 72 kDa band observed in mouse spleen lysates (calculated MW of 72.7 kDa band according to NP_848781.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Themis

THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling.

Long Name

Thymocyte-expressed Molecule Involved in Selection

Alternate Names

C6orf190, C6orf207, Gasp, SPOT, TSEPA

Entrez Gene IDs

210757 (Mouse)

Gene Symbol

THEMIS

UniProt

NP_848781.1 (Human)

Additional Themis Products

Product Documents for Themis Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Themis Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Themis Antibody

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