Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1072207
View all Theophylline Primary Antibodies »

Product Specifications

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Theophylline Antibody (1072207)

A competitive ELISA for detection of Theophylline.

A competitive ELISA for detection of Theophylline.

Biotinylated Theophylline (Tocris Catalog #8859) competes with Theophylline for binding to monoclonal mouse anti Theophylline (MAB11684) antibody coated on a plate @ 1ug/ml in PBS. Bound biotinylated- Theophylline was detected using Streptavidin-HRP (Catalog #DY998).

Applications for Human Theophylline Antibody (1072207)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Theophylline

Theophylline,  1,3-dimethylxanthine, is an oral methylxanthine bronchodilator for the treatment of asthma and chronic obstructive pulmonary disease (COPD). Methylxanthines are present in plants including Thea sinensis, Coffea arabica (coffee), and Theobroma cacao (coco, chocolate). Theophylline bronchodilatation mechanism is based on the inhibition of phosphodiesterase 3 (PDE3), and its anti inflammatory effects mediated via inhibition of phosphodiesterase 4 (PDE4) and activation of histone deacetylase-2 causing switching off activated inflammatory genes. Theophylline can also reverse corticosteroid resistance, alleviating asthma and COPD, symptoms.

Long Name

1,3-dimethyl-7H-purine-2,6-dione

Alternate Names

1,3 Dimethylxanthine, 58-55-9

Additional Theophylline Products

Product Documents for Human Theophylline Antibody (1072207)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Theophylline Antibody (1072207)

For research use only

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