Thromboxane B2 Parameter Assay Kit

R&D Systems | Catalog # KGE011

R&D Systems
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Key Product Details

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (50 µL)

Sensitivity

0.31 ng/mL

Assay Range

0.313-20 ng/mL (Cell Culture Supernates, Serum, Plasma, EDTA Plasma, Heparin Plasma, Urine)
View all Thromboxane B2 ELISA Kits »

Product Summary for Thromboxane B2 Parameter Assay Kit

The Parameter TXB2 Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure TXB2 in cell culture supernates, serum, plasma, and urine.

Product Specifications

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

Biotin

Species

Multi-Species

Specificity

Measures TXB2 levels in various samples

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 3.87 6.95 13.2 3.86 7.05 14.4
Standard Deviation 0.23 0.38 0.51 0.34 0.45 0.74
CV% 5.9 5.5 3.9 8.9 6.4 5.1

Recovery for Thromboxane B2 Parameter Assay Kit

The recovery of TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 88-110
EDTA Plasma (n=4) 94 86-105
Heparin Plasma (n=4) 97 87-107
Serum (n=4) 98 86-111
Urine (n=4) 98 84-111

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TXB2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Multi-species Thromboxane B2 ELISA Linearity

Scientific Data Images for Thromboxane B2 Parameter Assay Kit

Multi-species Thromboxane B2 ELISA Standard Curve

Multi-species Thromboxane B2 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Thromboxane B2

Thromboxane A2 (TXA2), is an eicosanoid involved in platelet aggregation, vasoconstriction, and reproductive functions. However, as TXA2 is rapidly degraded in vivo, TXA2 production is typically examined by assaying the more stable Thromboxane B2 (TXB2) or 11-dehydro-TXB2 (11-d-TXB2) derivatives. Levels of these metabolites are helpful markers in the study of diseases including liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and others.

Long Name

Thromboxane B2

Alternate Names

TXB2

Additional Thromboxane B2 Products

Product Documents for Thromboxane B2 Parameter Assay Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Thromboxane B2 Parameter Assay Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Thromboxane B2 Parameter Assay Kit

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Protocols

View specific protocols for Thromboxane B2 Parameter Assay Kit (KGE011):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. Calibrator Diluent
  3.   Add 150 µL of Calibrator Diluent to the non-specific binding (NSB) wells.
  4.   Add 100 µL of Calibrator Diluent to the zero standard (B0) wells.
    5. 100 µL Standard, Control, or Sample
  5.   Add 100 µL of Standard, control, or sample to the remaining wells.
    6. 50 µL Primary Antibody Solution
  6.   Add 50 µL of Primary Antibody Solution to each well (except the NSB wells).
  7.   Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker. Do not wash the plate.
    8. 50 µL Conjugate
  8.   Add 50 µL of Conjugate to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour on the shaker.
  9.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    10. 200 µL Substrate Solution
  10. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    11. 100 µL Stop Solution
  11. Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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