Tissue alpha-L-Fucosidase/FUCA1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-00209

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Tissue alpha-L-Fucosidase/FUCA1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Tissue alpha-L-Fucosidase/FUCA1 (Accession#: P04066; Gln32-Lys466)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Tissue alpha-L-Fucosidase/FUCA1 Antibody - Azide and BSA Free

Tissue alpha-L-Fucosidase/FUCA1 Antibody - Azide and BSA Free

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody [NBP3-00209] -

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody [NBP3-00209] - NBP3-00209 at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:54 kDa
Tissue alpha-L-Fucosidase/FUCA1 Antibody - Azide and BSA Free

Immunoprecipitation: Tissue alpha-L-Fucosidase/FUCA1 Antibody [NBP3-00209] -

Immunoprecipitation: Tissue alpha-L-Fucosidase/FUCA1 Antibody [NBP3-00209] - FUCA1 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 1 uL NBP3-00209 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00209, at 1:500 dilution. Secondary antibody: IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 54 kDa Observed band size: 54 kDa

Applications for Tissue alpha-L-Fucosidase/FUCA1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tissue alpha-L-Fucosidase/FUCA1

Alpha-L-fucosidase (EC 3.2.1.51) is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids (Occhiodoro et al., 1989 [PubMed 2803312]). At least 2 separate polymorphic alpha-L-fucosidases are recognized in man: that in tissues, FUCA1, which is deficient in fucosidosis (MIM 230000), and that in plasma, FUCA2 (MIM 136820).[supplied by OMIM]

Alternate Names

FUCA1, Tissue alphaLFucosidase

Gene Symbol

FUCA1

Additional Tissue alpha-L-Fucosidase/FUCA1 Products

Product Documents for Tissue alpha-L-Fucosidase/FUCA1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Tissue alpha-L-Fucosidase/FUCA1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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