TPR Antibody

Novus Biologicals | Catalog # NB100-2865

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TPR Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1650 and 1700 of human Translocated Promoter Region Protein using the numbering given in entry NP_003283.1 (GeneID 7175).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPR Antibody

Western Blot: TPR Antibody [NB100-2865]

Western Blot: TPR Antibody [NB100-2865]

Western Blot: TPR Antibody [NB100-2865] - Whole cell lysate (50 ug) from HeLa, Jurkat, K-562, Hep-G2, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TPR antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation: TPR Antibody [NB100-2865]

Immunoprecipitation: TPR Antibody [NB100-2865]

Immunoprecipitation: TPR Antibody [NB100-2865] - Detection of human TPR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TPR antibody NB100-2865(lot NB100-2865-2) used for IP at 6 ug per reaction. TPR was also immunoprecipitated by a previous lot of this antibody (lot NB100-2865-1) and rabbit anti-TPR antibody NB100-2866. For blotting immunoprecipitated TPR, NB100-2865 was used. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for TPR Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TPR

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias.

Alternate Names

nuclear pore complex-associated protein TPR, nucleoprotein TPR, translocated promoter region (to activated MET oncogene), tumor potentiating region

Entrez Gene IDs

7175 (Human)

Gene Symbol

TPR

UniProt

NP_003283.1 (Human)

Additional TPR Products

Product Documents for TPR Antibody

Certificate of Analysis

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Product Specific Notices for TPR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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