TRBP Antibody (1D9) - Azide and BSA Free

Novus Biologicals | Catalog # H00006895-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D9

Format

Azide and BSA Free
View all TRBP Primary Antibodies »

Product Specifications

Immunogen

TARBP2 (NP_599150, 141 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RSPPMELQPPVSPQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFIEIGSGTSKKLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVG

Specificity

TARBP2 - Tar (HIV-1) RNA binding protein 2 (1D9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TRBP Antibody (1D9) - Azide and BSA Free

Western Blot: TRBP Antibody (1D9) [H00006895-M03]

Western Blot: TRBP Antibody (1D9) [H00006895-M03]

Western Blot: TRBP Antibody (1D9) [H00006895-M03] - Analysis of TARBP2 expression in transfected 293T cell line by TARBP2 monoclonal antibody (M03), clone 1D9. Lane 1: TARBP2 transfected lysate (Predicted MW: 39 KDa). Lane 2: Non-transfected lysate.
Western Blot: TRBP Antibody (1D9) [H00006895-M03]

Western Blot: TRBP Antibody (1D9) [H00006895-M03]

Western Blot: TRBP Antibody (1D9) [H00006895-M03] - Analysis of TARBP2 transfected lysate using anti-TARBP2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TARBP2 purified rabbit polyclonal antibody.
ELISA: TRBP Antibody (1D9) [H00006895-M03]

ELISA: TRBP Antibody (1D9) [H00006895-M03]

ELISA: TRBP Antibody (1D9) [H00006895-M03] - Detection limit for recombinant GST tagged TARBP2 is approximately 0.1ng/ml as a capture antibody.

Applications for TRBP Antibody (1D9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TRBP

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.

Alternate Names

LOQS, RISC-loading complex subunit TARBP2, TAR (HIV) RNA binding protein 2, TAR (HIV) RNA-binding protein 2, TAR (HIV) RNA-binding protein TRBP1, TAR (HIV-1) RNA binding protein 2, TAR RNA binding protein 2, TAR RNA-binding protein 2, trans-activation responsive RNA-binding protein, Trans-activation-responsive RNA-binding protein, TRBP, TRBP1, TRBP2

Entrez Gene IDs

6895 (Human)

Gene Symbol

TARBP2

UniProt

NP_599150 (Human)

Additional TRBP Products

Product Documents for TRBP Antibody (1D9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TRBP Antibody (1D9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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