TRIM3/BERP Antibody

Novus Biologicals | Catalog # NB100-2494

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRIM3/BERP Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence AKREDSPGPEVQP-C corresponding to N-Terminus according to NP_150594.2.

Specificity

Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRIM3/BERP Antibody

Western Blot: TRIM3/BERP Antibody [NB100-2494]

Western Blot: TRIM3/BERP Antibody [NB100-2494]

Western Blot: TRIM3/BERP Antibody [NB100-2494] - Staining of Human Cerebellum (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: TRIM3/BERP Antibody [NB100-2494]

Western Blot: TRIM3/BERP Antibody [NB100-2494]

Western Blot: TRIM3/BERP Antibody [NB100-2494] - HEK293 overexpressing TRIM3 and probed with (mock transfection in first lane).
Western Blot: TRIM3/BERP Antibody [NB100-2494]

Western Blot: TRIM3/BERP Antibody [NB100-2494]

Western Blot: TRIM3/BERP Antibody [NB100-2494] - Western blot analysis of TRIM3/BERP in Mouse Brain lysate (RIPA buffer, 30ug total protein per lane) using NB100-2494 at 0.05ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for TRIM3/BERP Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 1 ug/ml
Application Notes
Please note the HEK cell experiements were performed using a previous batch (different goat). WB: Approx. 85 kDa band observed in mouse brain and human brain (cerebellum) lysate (predicted MW of approx. 80.8 kDa band according to NP_006449.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM3/BERP

Gene ID(s): 10612 (human)

Alternate Names

BERPRING finger protein 97, Brain-expressed RING finger protein, HAC1, RING finger protein 22, RNF22brain expressed ring finger, RNF97FLJ16135, tripartite motif containing 3, tripartite motif protein TRIM3, tripartite motif-containing 3, tripartite motif-containing protein 3

Entrez Gene IDs

10612 (Human); 55992 (Mouse); 83616 (Rat)

Gene Symbol

TRIM3

UniProt

NP_006449.2 (Human)

Additional TRIM3/BERP Products

Product Documents for TRIM3/BERP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TRIM3/BERP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRIM3/BERP Antibody

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