TRIM4 Antibody
Novus Biologicals | Catalog # NB100-1225
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-ASLVIPPVTDRK corresponding to C-Terminus according to NP_148977.1, NP_149082.1.
Specificity
This antibody is expected to recognise both reported human isoforms (as represented by NP_148977 and NP_149082).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for TRIM4 Antibody
Western Blot: TRIM4 Antibody [NB100-1225]
Western Blot: TRIM4 Antibody [NB100-1225] - Detection of TRIM4 in HepG2 lysate (RIPA buffer, 30 ug total protein per lane) using this antibody at 1 ug/ml.Applications for TRIM4 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
1 ug/ml
Application Notes
WB: Approx. 55-60 kDa band observed in HepG2 whole lysate (predicted MW of 58 kDa band and 55 kDa band according to NP_148977 and NP_149082).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TRIM4
Alternate Names
RING finger protein 87, RNF87tripartite motif protein 4, tripartite motif containing 4, tripartite motif-containing 4, tripartite motif-containing protein 4
Entrez Gene IDs
89122 (Human)
Gene Symbol
TRIM4
UniProt
Additional TRIM4 Products
Product Documents for TRIM4 Antibody
Certificate of Analysis
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Product Specific Notices for TRIM4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for TRIM4 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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