Troponin I Type 1 (slow skeletal) Antibody

Novus Biologicals | Catalog # NBP2-26202

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Troponin I Type 1 (slow skeletal) Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence PEVERKPKITASRK-C corresponding to N-Terminus according to NP_003272.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Troponin I Type 1 (slow skeletal) Antibody

Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP2-26202]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP2-26202]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP2-26202] - Staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Troponin I Type 1 (slow skeletal) Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human and mouse skeletal muscle lysates (calculated MW of 21.7 kDa band according to NP_003272.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Troponin I Type 1 (slow skeletal)

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. (provided by RefSeq)

Alternate Names

DKFZp451O223, slow-twitch isoform, SSTNI, TNN1, troponin I type 1 (skeletal, slow), troponin I, skeletal, slow, troponin I, slow skeletal muscle

Entrez Gene IDs

7135 (Rat)

Gene Symbol

TNNI1

Additional Troponin I Type 1 (slow skeletal) Products

Product Documents for Troponin I Type 1 (slow skeletal) Antibody

Certificate of Analysis

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Product Specific Notices for Troponin I Type 1 (slow skeletal) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Troponin I Type 1 (slow skeletal) Antibody

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