Trypsin Inhibitor Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-21629

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Key Product Details

Species Reactivity

Plant

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Trypsin Inhibitor Primary Antibodies »

Product Specifications

Immunogen

Trypsin Inhibitor [Soybean] (Uniprot: P01070)

Reactivity Notes

Cross reactivity against Trypsin Inhibitor from other sources may occur but have not been specifically determined.

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soybean]. Cross reactivity against Trypsin Inhibitor from other sources may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soybean]

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Trypsin Inhibitor Antibody - BSA Free

Western Blot: Trypsin Inhibitor Antibody [NBP2-21629]

Western Blot: Trypsin Inhibitor Antibody [NBP2-21629]

Western Blot: Trypsin Inhibitor Antibody [NBP2-21629] - To detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody
Western Blot: Trypsin Inhibitor Antibody [NBP2-21629]

Western Blot: Trypsin Inhibitor Antibody [NBP2-21629]

Western Blot: Trypsin Inhibitor Antibody [NBP2-21629] - Rabbit anti Soybean Trypsin Inhibitor antibody was used to detect purified Soybean Trypsin Inhibitor. Samples of ~1 and 0.25 ug of purified Soybean Trypsin Inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rabbit anti Soybean Trypsin Inhibitor antibody. Primary antibody was detected with Dylight 488 conjugated Streptavidin and Atto 425 conjugated goat anti rabbit and imaged on the VersaDoc imaging system.
Trypsin Inhibitor Antibody

Trypsin Inhibitor Antibody

Rabbit anti Soybean Trypsin Inhibitor antibody was used to detect purified Soybean Trypsin Inhibitor. Samples of ~1 and 0.25 ug of purified Soybean Trypsin Inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rabbit anti Soybean Trypsin Inhibitor antibody (200-4679 lot 6594 1:5K in, ON 4 C). Primary antibody was detected with Dylight 488 conjugated Streptavidin (S000-41 Lot 21091, 1:5K) and Atto 425 conjugated goat anti rabbit ( lot 26423C 1:10K 1.5 hr RT in ) and imaged on the BioRad VersaDoc imaging system.
Trypsin Inhibitor Antibody

Trypsin Inhibitor Antibody

Western Blot of Trypsin Inhibitor Antibody. Lane 1: Opal PreStained Molecular Weight Marker

Applications for Trypsin Inhibitor Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested by ELISA and western blot and is assayed against 1.0 ug of Trypsin Inhibitor [Soybean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Trypsin Inhibitor

The soybean trypsin inhibitor was first crystallized by Kunitz in 1945 and is one of several such inhibitors found in soybeans. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly inhibited by soy inhibitor.

Alternate Names

KTI3, Kunitz trypsin inhibitor

Gene Symbol

KTI3

UniProt

P01070 (Plant)

Additional Trypsin Inhibitor Products

Product Documents for Trypsin Inhibitor Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Trypsin Inhibitor Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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