TSLP ELISA Development Kit

Novus Biologicals | Catalog # DDXK-E-TSLP

Novus Biologicals
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Key Product Details

Assay Type

Sandwich ELISA

Assay Range

0.31 - 20ng/ml

Sample Type

Human cell culture supernatants, plasma, serum, and various biological fluids. (100 uL)

Sensitivity

1 ng/ml

Reactivity

Human
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Product Specifications

Sample Volume Required

100 uL

Conjugate

Unconjugated

Application Notes

This is an enzyme-linked immunosorbent assay for quantitative detection.

Scientific Data Images for TSLP ELISA Development Kit

ELISA: TSLP ELISA Development Kit [DDXK-E-TSLP]

ELISA: TSLP ELISA Development Kit [DDXK-E-TSLP]

ELISA: TSLP ELISA Kit [DDXK-E-TSLP] - Each laboratory should establish its own standard curve. Example of standard curve using serial dilutions of TSLP (20ng/ml) prepared from supernatants of TSLP-transfected eukaryotic cells.

Kit Contents for TSLP ELISA Development Kit

  • Capture Antibody: 0.5 mg/mL of mouse anti-TSLP monoclonal antibody (clone 209A2.03). Dilute in carbonate buffer pH9.6 to a working concentration of 2.5 ug /mL
  • Detection Antibody: 0.5mg/mL of Biotin-conjugated anti-TSLP mouse monoclonal antibody (clone 210H1.04). Dilute to a working concentration of 3ug/mL in PBS-BSA1%-Tween 0.05%.
  • Standard: Each vial contains 1ug/mL of recombinant TSLP produced, purified and concentrated from eukaryotic cells. A 7-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 20 ng /mL is recommended

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TSLP

TSLP (thymic stromal lymphopoietin) is a hemopoietic protein of 159 aa (18 kDa), produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromallike cells. It is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. It is also detected in the thymus and the tissue cells of the bone marrow. TSLP affects the transition from pre-B cells in B. Alternative splicing of this gene results in two transcripts variants. TSLP is proposed to signal through a heterodimeric receptor complex composed of TSLP-receptor and IL7R chain. Expressed in many tissues, it prevents apoptosis and stimulates the growth of myeloid cells. (Reche PA et al, J Immunol., 2001, 167(1):336-43; Dorshkind. K, Nature. Immunol., 2000 (1):369-370). Monoclonal antibodies raised against human TSLP were obtained after mice immunization with TSLP-His-tagged transfected 293T cells, and were used to set up a quantitative immunoassay to determine TSLP concentration in biological samples

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Gene Symbol

TSLP

Additional TSLP Products

Product Documents for TSLP ELISA Development Kit

Certificate of Analysis

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Product Specific Notices for TSLP ELISA Development Kit

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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