Tsukushi (TSK) is a secreted member of the SLRP (small leucine-rich proteoglycan) family of extracellular matrix molecules. It is named after a Japanese horsetail plant, which its expression pattern resembles in the chick primitive streak. Tsukushi binds BMP and Delta-1, contributes to formation of the primitive organizer and neural crest, and is upregulated in certain breast and bone cancer cell lines.
Tsukushi/TSK Antibody (462310) [Janelia Fluor® 646]
Novus Biologicals | Catalog # FAB6376J
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Janelia Fluor 646
Antibody Source
Monoclonal Rat IgG2C Clone # 462310
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Tsukushi/TSK
Thr18-Leu354
Accession # Q8CBR6
Thr18-Leu354
Accession # Q8CBR6
Specificity
Detects mouse Tsukushi/TSK in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human Tsukushi/TSK is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2C
Applications for Tsukushi/TSK Antibody (462310) [Janelia Fluor® 646]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Tsukushi/TSK
Long Name
Tsukushin Leucine-rich Repeat Containing Protein 54
Alternate Names
E2IG4, LRRC54, TSKU
Gene Symbol
TSKU
Additional Tsukushi/TSK Products
Product Documents for Tsukushi/TSK Antibody (462310) [Janelia Fluor® 646]
Product Specific Notices for Tsukushi/TSK Antibody (462310) [Janelia Fluor® 646]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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