Tumor-Derived Exosome Isolation Beads

Novus Biologicals | Catalog # NBP3-14798

Novus Biologicals
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Key Product Details

Species

Human

Applications

ELISA

Product Summary for Tumor-Derived Exosome Isolation Beads

Tumor-derived Exosome Immunobeads are 0.4 um latex beads covalently coupled with antibodies that allows enrichment in Tumor-derived exosomes.

Using the supplied Elution Buffer, EVs can be eluted from Immunocapture beads and used in downstream analyses such as protein profiling and nucleic acid extraction.

Immunobeads can be re-used after incubation with Bead Regeneration Buffer.

Shelf life of 8 months.

Product Specifications

Sample Volume

0.5 -1 mL

Scientific Data Images for Tumor-Derived Exosome Isolation Beads

Tumor-Derived Exosome Isolation Beads

Tumor-Derived Exosome Isolation Beads [NBP3-14798]

Tumor-Derived Exosome Isolation Beads [NBP3-14798] - Anti-Alix WB analysis on exosomes immunocaptured with beads for overall (NBP3-11768) and for tumor-derived exosomes (NBP3-14798). WB shows the capture of exosomes only for cancer patients when beads for tumor-derived exosomes are used.
Tumor-Derived Exosome Isolation Beads

Tumor-Derived Exosome Isolation Beads [NBP3-14798]

Tumor-Derived Exosome Isolation Beads [NBP3-14798] - Alix expression by western blotting of human plasma exosomes captured on lmmunobeads from 0.5 ml of plasma in comparison with exosomes purified via ultracentrifuge. 1, 3 and 5: lmmunocapture with immunobeads from 1 ml, 0.5 ml and 100 µ1 of human plasma respectively. 2, 4, 6: Ultra centrifuged exosomes from plasma after immunobeads capture. 7 lmmunocapture with immunobeads isotype coupled. 8, 9: Ultracentifuged exosome from 0.5 ml and 1 ml of human plasma.

Kit Contents for Tumor-Derived Exosome Isolation Beads

  • Bead Regeneration Buffer
  • Exosome Elution Buffer
  • Tumor-derived Exosome Immunocapture Beads

Formulation, Preparation, and Storage

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C.

Background: Tumor-Derived Exosome Isolation Beads

Immunocapture for isolation of exosomes relies on the antigen-antibody interaction. Antibodies directed at target of interest are coated either in a 96-well plate or conjugated to a bead. The sample is applied directly into coated well, or into sample with coated beads, and EVs are allowed to bind. In a final step, EVs can be eluted from the antibody and used in downstream functional and phenotypic analysis. This method of isolation is highly specific, resulting in extremely pure EV populations.

Alternate Names

EV isolation, exosome isolation, extracellular vesicle isolation

Additional Tumor-Derived Exosome Isolation Beads Products

Product Documents for Tumor-Derived Exosome Isolation Beads

Certificate of Analysis

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Product Specific Notices for Tumor-Derived Exosome Isolation Beads

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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