USP14 Antibody (1F8) - Azide and BSA Free

Novus Biologicals | Catalog # H00009097-M08

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F8

Format

Azide and BSA Free
View all USP14 Primary Antibodies »

Product Specifications

Immunogen

USP14 (NP_005142, 395 a.a. ~ 494 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ

Specificity

USP14 - ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) (1F8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for USP14 Antibody (1F8) - Azide and BSA Free

Western Blot: USP14 Antibody (1F8) [H00009097-M08]

Western Blot: USP14 Antibody (1F8) [H00009097-M08]

Western Blot: USP14 Antibody (1F8) [H00009097-M08] - Analysis of USP14 expression in Raw 264.7 (Cat # L024V1).
Western Blot: USP14 Antibody (1F8) [H00009097-M08]

Western Blot: USP14 Antibody (1F8) [H00009097-M08]

Western Blot: USP14 Antibody (1F8) [H00009097-M08] - Analysis of USP14 expression in PC-12 (Cat # L012V1).
Western Blot: USP14 Antibody (1F8) [H00009097-M08]

Western Blot: USP14 Antibody (1F8) [H00009097-M08]

Western Blot: USP14 Antibody (1F8) [H00009097-M08] - Analysis of USP14 expression in Hela S3 NE (Cat # L013V3).
Western Blot: USP14 Antibody (1F8) [H00009097-M08]

Western Blot: USP14 Antibody (1F8) [H00009097-M08]

Western Blot: USP14 Antibody (1F8) [H00009097-M08] - Analysis of USP14 expression in NIH/3T3 (Cat # L018V1).
ELISA: USP14 Antibody (1F8) [H00009097-M08]

ELISA: USP14 Antibody (1F8) [H00009097-M08]

ELISA: USP14 Antibody (1F8) [H00009097-M08] - Detection limit for recombinant GST tagged USP14 is approximately 1ng/ml as a capture antibody.

Applications for USP14 Antibody (1F8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: USP14

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Ubiquitin Specific Protease 14

Alternate Names

TGT, Ubiquitin Thioesterase 14, Ubp6

Entrez Gene IDs

9097 (Human)

Gene Symbol

USP14

UniProt

NP_005142 (Human)

Additional USP14 Products

Product Documents for USP14 Antibody (1F8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for USP14 Antibody (1F8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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