Viral Chikungunya Virus E1 Antibody Summary
Accession # Q8JUX5
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Chikungunya Virus E1 by Western Blot. Western blot shows lysates of recombinant Chikungunya Virus (CHIKV) E1. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97792) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Chikungunya Virus E1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chikungunya Virus E1
Chikungunya fever is a pandemic disease caused by the flavivirus Chikungunya (CHIKV). The 47 kDa E1 glycoprotein mediates viral membrane fusion during CHIKV infection. The CHIKV envelope protein E1 is a component of the viral spike, which is composed of triplets of heterodimer of E1 and E2 glycoproteins, expressed on the viral surface. The viral spike proteins facilitate attachment to cell surfaces and viral entry into the cells. The E1 envelope protein is a class II fusion protein that mediates low pH-triggered membrane fusion during virus infection.
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