Viral Japanese Encephalitis virus NS1 Antibody (995010)

R&D Systems | Catalog # MAB10006

R&D Systems

Key Product Details

Species Reactivity

Validated:

Viral

Cited:

Primate - Cercopithecus aethiops (African Green Monkey)

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 995010
View all Japanese Encephalitis virus NS1 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived Japanese Encephalitis virus NS1
Thr796-Ala1146
Accession # P32886

Specificity

Detects Japanese Encephalitis virus NS1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Viral Japanese Encephalitis virus NS1 Antibody (995010)

Detection of Japanese Encephalitis virus NS1 antibody by Western Blot.

Detection of Japanese Encephalitis virus NS1 by Western Blot.

Western blot shows recombinant Japanese Encephalitis virus NS1 (rvJEV NS1). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB10006) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Japanese Encephalitis virus NS1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Viral Japanese Encephalitis virus NS1 Antibody (995010)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Japanese Encephalitis virus NS1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Japanese Encephalitis virus NS1

Japanese encephalitis virus (JEV) non-structural protein 1 (NS1) is produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the leading cause of virus-induced encephalitis globally (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral JEV NS1 contains 352 amino acids and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

References

  1. Nain, M. et al. (2016) Rev. Med. Virol. 26:129.
  2. Rastogi, M. et al. (2016) Virol. J. 13:131.

Alternate Names

JE virus, JEV, JEV-NS1, JEVgp1

Entrez Gene IDs

1489713 (Viral)

Gene Symbol

POLY

UniProt

P32886

Additional Japanese Encephalitis virus NS1 Products

Product Documents for Viral Japanese Encephalitis virus NS1 Antibody (995010)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral Japanese Encephalitis virus NS1 Antibody (995010)

For research use only

Citations for Viral Japanese Encephalitis virus NS1 Antibody (995010)

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