Bovine Vitamin B12 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60195

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.299 pg/mL

Assay Range

0.312 pg/mL - 20 pg/mL
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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Bovine

Specificity

This assay has high sensitivity and excellent specificity for detection of bovine VB12. No significant cross-reactivity or interference between bovine VB12 and analogues was observed.

Description

Assay Length: 1-5 hours

精度

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Bovine Vitamin B12 ELISA Kit (Colorimetric)

Bovine Vitamin B12 ELISA Kit (Colorimetric)

Bovine Vitamin B12 ELISA Kit (Colorimetric) [NBP2-60195] -

Bovine Vitamin B12 ELISA Kit (Colorimetric) [NBP2-60195] - Standard Curve Reference

Kit Contents for Bovine Vitamin B12 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • Biotin-conjugate (10 x concentrate)
  • Biotin-conjugate Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Vitamin B12

This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Additional Vitamin B12 Products

Product Documents for Bovine Vitamin B12 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Bovine Vitamin B12 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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