WAPL Antibody (2B3) - Azide and BSA Free

Novus Biologicals | Catalog # H00023063-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2B3

Format

Azide and BSA Free
View all WAPL Primary Antibodies »

Product Specifications

Immunogen

KIAA0261 (AAH17393, 1 a.a. ~ 402 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Reactivity Notes

Human. Other species not tested.

Specificity

KIAA0261 (2B3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for WAPL Antibody (2B3) - Azide and BSA Free

Western Blot: WAPL Antibody (2B3) [H00023063-M02]

Western Blot: WAPL Antibody (2B3) [H00023063-M02]

Western Blot: WAPL Antibody (2B3) [H00023063-M02] - Analysis of KIAA0261 expression in transfected 293T cell line by KIAA0261 monoclonal antibody (M02), clone 2B3.Lane 1: KIAA0261 transfected lysate(45.6 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: WAPL Antibody (2B3) [H00023063-M02]

Immunoprecipitation: WAPL Antibody (2B3) [H00023063-M02]

Immunoprecipitation: WAPL Antibody (2B3) [H00023063-M02] - Analysis of WAPAL transfected lysate using anti-WAPAL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WAPAL monoclonal antibody.
Sandwich ELISA: WAPL Antibody (2B3) [H00023063-M02] - Detection limit for recombinant GST tagged KIAA0261 is approximately 0.3ng/ml as a capture antibody.

Applications for WAPL Antibody (2B3) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WAPL

The hWAPL mRNA was predominantly expressed in uterine cervical cancer, with weak expression in all other normal and tumor tissues examined. hWAPL expression in benign epithelia was confined to the basal cell layers, whereas in dysplasias it increasingly appeared in more superficial cell layers and showed a significant correlation with severity of dysplasia. Diffuse hWAPL expression was found in all invasive squamous cell carcinomas examined. These results demonstrate that hWAPL is associated with cell growth, and the hWAPL expression may play a significant role in cervical carcinogenesis and tumor progression.

Alternate Names

FOEKIAA0261friend of EBNA2 (Epstein-Barr virus nuclear protein 2), Friend of EBNA2 protein, WAPLwings apart-like protein homolog, wings apart-like homolog (Drosophila)

Entrez Gene IDs

23063 (Human)

Gene Symbol

WAPL

UniProt

AAH17393 (Human)

Additional WAPL Products

Product Documents for WAPL Antibody (2B3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for WAPL Antibody (2B3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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