ZCRB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-37757

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZCRB1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 155 to 205 of human Zinc Finger CCHC-type and RNA Binding Motif 1 using the numbering given in entry NP_149105.3 (GeneID 85437).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZCRB1 Antibody - BSA Free

Western Blot: ZCRB1 Antibody [NBP2-37757]

Western Blot: ZCRB1 Antibody [NBP2-37757]

Western Blot: ZCRB1 Antibody [NBP2-37757] - Detection of Human ZCRB1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCRB1 antibody used for IP at 6 ug per reaction. ZCRB1 was also immunoprecipitated by rabbit anti-ZCRB1 antibody. For blotting immunoprecipitated ZCRB1, NBP2-37757 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: ZCRB1 Antibody [NBP2-37757]

Western Blot: ZCRB1 Antibody [NBP2-37757]

Western Blot: ZCRB1 Antibody [NBP2-37757] - Detection of Human and Mouse ZCRB1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCRB1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for ZCRB1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZCRB1

Pre-mRNA splicing is catalyzed by the spliceosome. U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5' splice site and branch-point sequence. U11 and U12 snRNPs are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron. The protein encoded by this gene contains a RNA recognition motif. It was identified as one of the protein components of U11/U12 snRNPs. This protein and many other U11/U12 snRNP proteins are highly conserved in organisms known to contain U12-type introns. These proteins have been shown to be essential for cell viability, suggesting the key roles in U12-type splicing. [provided by RefSeq]

Alternate Names

MADP-1, MADP1U11/U12 snRNP 31 kDa protein, MGC26805, RBM36, U11/U12 small nuclear ribonucleoprotein 31 kDa protein, U11/U12 snRNP 31K, U11/U12-31K, ZCCHC19, zinc finger CCHC-type and RNA binding motif 1, zinc finger CCHC-type and RNA-binding motif-containing protein 1

Gene Symbol

ZCRB1

UniProt

NP_149105.3 (Human)

Additional ZCRB1 Products

Product Documents for ZCRB1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ZCRB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ZCRB1 Antibody - BSA Free

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