This study evaluates Wnt/R-Spondin (RSPO) agonists and a defined, animal-free synthetic matrix as alternatives to traditional components used in human intestinal organoid culture. Traditionally, organoid growth depends on Wnt-3a and R-Spondin proteins along with animal-derived matrices like basement membrane extract (BME).
Key findings:
- Wnt/RSPO1-3 agonists support enteroid organoid culture
- Wnt/RSPO1-2 agonists demonstrate higher bioactivity and drive greater organoid growth at lower concentrations
- Cultrex Synthetic Hydrogel supports robust enteroid organoid expansion and differentiation with Wnt/RSPO2 agonist
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