Cell Culture Media
Improve the consistency of your cell therapy manufacturing process by taking an important step towards defined cell culture conditions.
R&D Systems™ cell culture media are designed to support the in vitro cultivation of cell lines, primary mammalian cells, and advanced cell culture models. With over 30 years of experience, each production lot of cell culture media is thoroughly tested to ensure the superior quality and lot-to-lot consistency that researchers worldwide expect from our highly bioactive recombinant proteins.
ExCellerate Cell Expansion Media provide an optimized serum-free culture environment for the generation and expansion of individual immune cell types and induced pluripotent stem cells (iPSCs).
- Robust expansion and maintenance of cell cultures
- Reduce variability in your cell cultures
- Streamline comparability testing if raw material changes are required
- Cross-reference Drug Master Files to facilitate regulatory compliance
- Reduce time-to-market for your cell therapy
Immune Cell Expansion Media
Mitigating risk and embedding flexibility into your cell culture process will improve process reproducibility and accelerate your immune cell therapy development. These ExCellerate cell expansion media are optimized for specific cell types and are key to building a culture process that ensures high yields and high quality.
ExCellerate Human NK Cell Expansion Media
- Made from completely animal-free raw materials
- Serum-free and phenol red-free formulation
- Maintains high fold expansion and killing capacity
- Enables restimulation without exhaustion
GMP Human T Cell Media
- Clinic-ready formulation
- Robust T cell expansion from PBMCs or purified T cells
- Adopted by academic and biopharma clinical programs
Pluripotent Stem Cell Expansion Media
Achieve robust expansion and extended maintenance of your stem cells in well-defined culture media. ExCellerate iPSC Expansion Media sustains cell viability, genotype, and multi-lineage differentiation potential/pluripotency over long term culture and is compatible with various matrices and cell lines. Seamlessly transition to clinical applications with equivalent preclinical RUO and GMP-grade media.
ExCellerate GMP and Preclinical RUO iPSC Expansion Media
- Animal component-free, all-in-one formulation
- Compatible with a variety of matrices and cell lines
- Maintains stable cell integrity over long-term culture
- GMP media is manufactured in an animal-free facility with ISO:9001 and ISO:13485 certifications
Available Media and Supplements
Immune Cell Expansion Media | |||
|---|---|---|---|
| ExCellerate B Cell Media | CCM031 (Xeno-Free) | ||
| ExCellerate NK Cell Expansion Media | CCM032 (Xeno-Free) | CCM037 (Animal Component-Free) | |
| Human T Cell Expansion Media | CCM030 (Xeno-Free) | CCM038-GMP-1B (GMP, Bag) | CCM038-GMP-1L (GMP, Bottle) |
Hematopoietic Stem Cell Media | |||
|---|---|---|---|
| Human Methylcellulose Media | HSC002 (Base Media) | HSC003 (Complete Media) | HSC004 (Complete, Without Epo) |
| HSC005 (Enriched Media) | HSC005SF (Enriched, Serum-Free) | ||
| Mouse Methylcellulose Media | HSC006 (Base Media) | HSC007 (Complete Media) | |
Stem Cell Differentiation and Expansion Media | |||
|---|---|---|---|
| ExCellerate iPSC Expansion Media | CCM036 (Animal Component-Free) | CCM036-GMP (GMP, Animal-Free) | |
| Endothelial Cell Growth Media | CCM027 | ||
| Mouse Embryonic Fibroblast (MEF) Conditioned Media | AR005 | ||
| StemXVivo Chondrogenic Base Media | CCM005 | ||
| StemXVivo Dendritic Cell Base Media | CCM003 (Serum-Free) | ||
| StemXVivo MSC Expansion Media | CCM004 | CCM014 (Serum-Free) | CCM021 (Xeno-Free) |
| StemXVivo MSC Freezing Media | CCM016 (Serum-Free) | ||
| StemXVivo Osteogenic/Adipogenic Base Media | CCM007 | ||
N-2 and N21-MAX Media Supplements
N-2 media supplements are optimized for neural progenitor cells and their differentiated derivatives. Improved over published N1 formulation.
N21-MAX standard and insulin-free media supplements are optimized for neuronal cell cultures. Vitamin A-free is optimized for neural cell cultures and limits retinoic acid production to reduce unwanted differentiation. Improved over published B27/NS21 formulation.
Xeno-Free vs. Animal Component-Free Culture Media
Xeno-free media do not contain any non-human animal-derived components. Xeno-free media may contain human-derived components such as serum, holo-transferrin, or insulin. Animal component-free media do not contain any animal- or human-derived components. Elimination of these components further improves culture consistency compared to xeno-free media.
Benefits of Serum-Free Cell Culture
Using serum-free media eliminates variability from trace animal components in your culture media and reduces the risk of inconsistent results. Cultures expanded in these media are unaffected by the presence of unknown levels of vitamins, hormones, and growth factors in serum. Read The Importance of Serum- and Animal-Free Media for Stem Cell-Based Therapeutics Article at RegMedNet.
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Additional Resources
- Organoid Culture Protocols
- Stem Cell Protocols
- Immune Cell Protocols
- The Organoid Culture Handbook
- Evolution of Cell Culture Model Systems eBook
- Immune Cell Culture & Analysis Brochure
- Enhance Your TIL Expansion with ExCellerate T Cell Media
- Organoids and 3D Culture
- Proteins for Immune Cell Culture
- Cultrex™ Basement Membrane Extracts