10-DEBC hydrochloride

Tocris Bioscience | Catalog # 2558

Selective Akt/PKB inhibitor
Tocris Bioscience

Key Product Details

Description

Selective Akt/PKB inhibitor

Alternative Names

Akt Inhibitor X
View all Akt (Protein Kinase B) Inhibitors »

Product Description

10-DEBC hydrochloride is a selective inhibitor of Akt/PKB. Inhibits IGF-1-stimulated phosphorylation and activation of Akt (complete inhibition at 2.5 μM), suppressing downstream activation of mTOR, p70 S6 kinase and S6 ribosomal protein. Shows no activity at PDK1, SGK1 or PI 3-kinase. Inhibits cell growth (IC50 ~ 2-6 μM) and induces apoptosis in rhabdomyosarcoma cells.

Product Specifications for 10-DEBC hydrochloride

Molecular Weight

381.34

Formula

C20H25N2OCl.HCl

Storage

Desiccate at +4°C

Purity

≥98% (HPLC)

Chemical Name

10-[4'-(N,N-Diethylamino)butyl]-2-chlorophenoxazine hydrochloride

CAS Number

925681-41-0

PubChem ID

16760284

InChI Key

SVKSJUIYYCQZEC-UHFFFAOYSA-N

SMILES

Cl.CCN(CC)CCCCN1C2=CC=CC=C2OC2=C1C=C(Cl)C=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 38.13 100
DMSO 38.13 100

Preparing Stock Solutions for 10-DEBC hydrochloride

The following data is based on the product molecular weight 381.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.62 mL 13.11 mL 26.22 mL
5 mM 0.52 mL 2.62 mL 5.24 mL
10 mM 0.26 mL 1.31 mL 2.62 mL
50 mM 0.05 mL 0.26 mL 0.52 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 8 publications citing the usage of this product.

Product Documents for 10-DEBC hydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 10-DEBC hydrochloride

For research use only

Citations for 10-DEBC hydrochloride

Customer Reviews for 10-DEBC hydrochloride (1)

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  • Inhibition of Akt/PKB in BV2 microglia cells
    Name: Joanna Plastira
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: BV2 microglia cells
    Verified Customer | Posted 06/08/2019
    As part of our current study, we investigated the role of AKT pathway on the LPA induced inflammatory phenotype in microglia (downstream signaling). BV2 cells were incubated for different time points with LPA (1µM) in the presence or absence of AKT X (5µM). AKT inhibition totally decreased the expression of 2 pro-inflammatory transcription factors (pSTAT1 and pp65). The inhibitor was diluted in water.
    10-DEBC hydrochloride 2558

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