Basement membrane extract (BME) is a type of extracellular matrix (ECM) derived from Engelbreth-Holm-Swarm mouse sarcomas. BME is commonly used in cell culture and tissue engineering to provide a 2-D or 3-D scaffold for cells, allowing them to grow, differentiate, and interact in an environment that closely mimics the native ECM. This makes BME invaluable for modeling the behavior of cells in vivo. All Cultrex BME formulations are rigorously tested to ensure performance, quality, and lot-to-lot consistency.
Cultrex Spheroid Formation Extracellular Matrix, 10X
R&D Systems | Catalog # 3500-096-01
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Key Product Details
Features
Provides specialized extracellular matrix optimized to drive aggregation and/or spheroid formation of cells as a part of the Cultrex 3-D Culture Spheroid Cell Invasion Assay.
Species
Mouse
Product Summary for Cultrex Spheroid Formation Extracellular Matrix, 10X
Why Use Cultrex Spheroid Formation Extracellular Matrix, 10X?
The Cultrex 10X Spheroid Formation Extracellular Matrix (ECM) is a proprietary mixture of extracellular matrix proteins derived from murine EHS sarcoma and optimized for spheroid formation. The diluted ECM is a fluid in culture that promotes cell-cell adhesion within a low adhesion environment.
Negative for the presence of bacteria and fungi.
Negative for the presence of bacteria and fungi.
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Product Specifications
Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Endotoxin Level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing
Performance tested in Cultrex 3-D Spheroid Cell Invasion Assay - supports spheroid formation for human breast cancer
cells (MDA-MB-231) over a 72 hour period.
Scientific Data Images for Cultrex Spheroid Formation Extracellular Matrix, 10X
Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays.
MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay (Catalog # 3500-096-K). Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix (Catalog # 3500-096-01). Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix (Catalog # 3500-096-03). After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Stability
Product is stable for a minimum of 3 months from date of receipt when stored at -70 °C
Background: Basement Membrane Extracts
Additional Basement Membrane Extracts Products
Product Documents for Cultrex Spheroid Formation Extracellular Matrix, 10X
Certificate of Analysis
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Citations for Cultrex Spheroid Formation Extracellular Matrix, 10X
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