Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well Summary
Why Use Cultrex 3-D Spheroid Basememnt Membrane Extract Cell Invasion Assay, 96-well?
The Cultrex 3-D Culture Spheroid Cell Invasion Assay utilizes a 3-D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation Extracellular Matrix to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid is embedded in an invasion matrix composed of basement membrane proteins. This matrix forms a hydrogel network on which invasive cells can travel. Invasion modulating agents can be applied to the system to evaluate the impact on cell response. Cell invasion is visualized microscopically and can be quantified through image analysis software.
• Cultrex Spheroid Formation Matrix
• 3-D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals
• Cultrex Spheroid Invasion Matrix
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
For research use only. Not for diagnositic use.
Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay. Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix. Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix. After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.
Citations for Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Co-delivery of IKBKE siRNA and cabazitaxel by hybrid nanocomplex inhibits invasiveness and growth of triple-negative breast cancer
Authors: Z Zhao, Y Li, H Liu, A Jain, PV Patel, K Cheng
Sci Adv, 2020;6(29):eabb0616. 2020
Integrated pharmaco-proteogenomics defines two subgroups in isocitrate dehydrogenase wild-type glioblastoma with prognostic and therapeutic opportunities
Authors: S Oh, J Yeom, HJ Cho, JH Kim, SJ Yoon, H Kim, JK Sa, S Ju, H Lee, MJ Oh, W Lee, Y Kwon, H Li, S Choi, JH Han, JH Chang, E Choi, J Kim, NG Her, SH Kim, SG Kang, E Paek, DH Nam, C Lee, HS Kim
Nat Commun, 2020;11(1):3288. 2020
PAICS, a Purine Nucleotide Metabolic Enzyme, is Involved in Tumor Growth and the Metastasis of Colorectal Cancer
Authors: S Agarwal, BVSK Chakravart, M Behring, HG Kim, DS Chandrashe, N Gupta, P Bajpai, A Elkholy, SAH Balasubram, C Hardy, SA Diffalha, S Varambally, U Manne
Cancers (Basel), 2020;12(4):. 2020
Nitidine Chloride Is a Potential Alternative Therapy for Glioma Through Inducing Endoplasmic Reticulum Stress and Alleviating Epithelial-Mesenchymal Transition
Authors: Z Chen, J Zhang, H Xue, M Qian, X Guo, X Gao, J Xu, Y Qi, X Sun, G Li
Integr Cancer Ther, 2020;19(0):1534735419900. 2020
Targeting P4HA1 with a Small Molecule Inhibitor in a Colorectal Cancer PDX Model
Authors: S Agarwal, M Behring, HG Kim, P Bajpai, BVSK Chakravart, N Gupta, A Elkholy, S Al Diffalh, S Varambally, U Manne
Transl Oncol, 2020;13(4):100754. 2020
Hypoxia-induced lncRNA PDIA3P1 promotes mesenchymal transition via sponging of miR-124-3p in glioma
Authors: S Wang, Y Qi, X Gao, W Qiu, Q Liu, X Guo, M Qian, Z Chen, Z Zhang, H Wang, J Xu, H Xue, X Guo, P Zhang, R Zhao, G Li
Cell Death Dis, 2020;11(3):168. 2020
Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-&kappaB activation
Authors: J Xu, Z Zhang, M Qian, S Wang, W Qiu, Z Chen, Z Sun, Y Xiong, C Wang, X Sun, R Zhao, H Xue, G Li
J. Exp. Clin. Cancer Res., 2020;39(1):59. 2020
Aspartate &beta-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway
Authors: K Ogawa, Q Lin, L Li, X Bai, X Chen, H Chen, R Kong, Y Wang, H Zhu, F He, Q Xu, L Liu, M Li, S Zhang, K Nagaoka, R Carlson, H Safran, K Charpentie, B Sun, J Wands, X Dong
J Hematol Oncol, 2019;12(1):144. 2019
ASPH-notch Axis guided Exosomal delivery of Prometastatic Secretome renders breast Cancer multi-organ metastasis
Authors: Q Lin, X Chen, F Meng, K Ogawa, M Li, R Song, S Zhang, Z Zhang, X Kong, Q Xu, F He, X Bai, B Sun, MC Hung, L Liu, J Wands, X Dong
Mol. Cancer, 2019;18(1):156. 2019
Bacopasides I and II Act in Synergy to Inhibit the Growth, Migration and Invasion of Breast Cancer Cell Lines
Authors: HM Palethorpe, E Smith, Y Tomita, M Nakhjavani, AJ Yool, TJ Price, JP Young, AR Townsend, JE Hardingham
Molecules, 2019;24(19):. 2019
MicroRNA-214 targets PTK6 to inhibit tumorigenic potential and increase drug sensitivity of prostate cancer cells
Authors: P Cagle, S Niture, A Srivastava, M Ramalinga, R Aqeel, L Rios-Colon, U Chimeh, S Suy, SP Collins, R Dahiya, D Kumar
Sci Rep, 2019;9(1):9776. 2019
Low-dose zoledronate for the treatment of bone metastasis secondary to prostate cancer
Authors: E Akoury, P Ahangar, A Nour, J Lapointe, KP Guérard, L Haglund, DH Rosenzweig, MH Weber
Cancer Cell Int., 2019;19(0):28. 2019
Isoliquiritin Apioside Suppresses in vitro Invasiveness and Angiogenesis of Cancer Cells and Endothelial Cells
Authors: A Kim, JY Ma
Front Pharmacol, 2018;9(0):1455. 2018
Estradiol promotes breast cancer cell migration via recruitment and activation of neutrophils
Authors: G Vazquez Ro, A Abrahamsso, LD Jensen, C Dabrosin
Cancer Immunol Res, 2017;0(0):. 2017
Repositioning Bazedoxifene as a novel IL-6/GP130 signaling antagonist for human rhabdomyosarcoma therapy
Authors: H Xiao, HK Bid, X Chen, X Wu, J Wei, Y Bian, C Zhao, H Li, C Li, J Lin
PLoS ONE, 2017;12(7):e0180297. 2017
Musashi-2 (MSI2) supports TGF-beta signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis.
Authors: Kudinov A, Deneka A, Nikonova A, Beck T, Ahn Y, Liu X, Martinez C, Schultz F, Reynolds S, Yang D, Cai K, Yaghmour K, Baker K, Egleston B, Nicolas E, Chikwem A, Andrianov G, Singh S, Borghaei H, Serebriiskii I, Gibbons D, Kurie J, Golemis E, Boumber Y
Proc Natl Acad Sci U S A, 2016;113(25):6955-60. 2016
Tumor Cell Invasion Can Be Blocked by Modulators of Collagen Fibril Alignment That Control Assembly of the Extracellular Matrix.
Authors: Grossman M, Ben-Chetrit N, Zhuravlev A, Afik R, Bassat E, Solomonov I, Yarden Y, Sagi I
Cancer Res, 2016;76(14):4249-58. 2016
RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs
Authors: Hiroaki Kajiho
EMBO Rep, 2016;0(0):. 2016
Binding of 14-3-3 reader proteins to phosphorylated DNMT1 facilitates aberrant DNA methylation and gene expression.
Authors: Esteve P, Zhang G, Ponnaluri V, Deepti K, Chin H, Dai N, Sagum C, Black K, Correa I, Bedford M, Cheng X, Pradhan S
Nucleic Acids Res, 2015;0(0):. 2015
The Epstein-Barr virus causes epithelial-mesenchymal transition in human corneal epithelial cells via Syk/src and Akt/Erk signaling pathways.
Authors: Park G, Kim D, Kim Y, Kim S, Lee H, Yang J, Hur D
Invest Ophthalmol Vis Sci, 2014;55(3):1770-9. 2014
Multimodular biosensors reveal a novel platform for activation of G proteins by growth factor receptors.
Authors: Midde K, Aznar N, Laederich M, Ma G, Kunkel M, Newton A, Ghosh P
Proc Natl Acad Sci U S A, 0;112(9):E937-46. 0
The oncogenic triangle of HMGA2, LIN28B and IGF2BP1 antagonizes tumor-suppressive actions of the let-7 family.
Authors: Busch B, Bley N, Muller S, Glass M, Misiak D, Lederer M, Vetter M, Strauss H, Thomssen C, Huttelmaier S
Nucleic Acids Res, 0;44(8):3845-64. 0
CXCL16 signaling mediated macrophage effects on tumor invasion of papillary thyroid carcinoma.
Authors: Cho S, Kim Y, Sun H, Kim Y, Oh B, Yi K, Park D, Park Y
Endocr Relat Cancer, 0;23(2):113-24. 0
Endophilin A2 Promotes TNBC Cell Invasion and Tumor Metastasis.
Authors: Baldassarre T, Watt K, Truesdell P, Meens J, Schneider M, Sengupta S, Craig A
Mol Cancer Res, 0;13(6):1044-55. 0
The GAS6-AXL signaling network is a mesenchymal (Mes) molecular subtype-specific therapeutic target for ovarian cancer.
Authors: Antony J, Tan T, Kelly Z, Low J, Choolani M, Recchi C, Gabra H, Thiery J, Huang R
Sci Signal, 0;9(448):ra97. 0
The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors.
Authors: Lin A, Hu Q, Li C, Xing Z, Ma G, Wang C, Li J, Ye Y, Yao J, Liang K, Wang S, Park P, Marks J, Zhou Y, Zhou J, Hung M, Liang H, Hu Z, Shen H, Hawke D, Han L, Zhou Y, Lin C, Yang L
Nat Cell Biol, 0;19(3):238-251. 0
The effect of bone morphogenetic protein-2 on osteosarcoma metastasis.
Authors: Gill J, Connolly P, Roth M, Chung S, Zhang W, Piperdi S, Hoang B, Yang R, Guzik H, Morris J, Gorlick R, Geller D
PLoS ONE, 0;12(3):e0173322. 0
Regulation of extracellular matrix degradation and metastatic spread by IQGAP1 through endothelin-1 receptor signaling in ovarian cancer.
Authors: Chellini L, Caprara V, Spadaro F, Sestito R, Bagnato A, Rosano L
Matrix Biol, 0;0(0):. 0
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