Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well

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Spheroid Cell Invasion Assay_3500-096-K
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Citations (31)
Reviews (1)

Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well Summary

Provides a more complete and physiologically predictive cancer invasion model.

Why Use Cultrex 3-D Spheroid Basememnt Membrane Extract Cell Invasion Assay, 96-well?

The Cultrex 3-D Culture Spheroid Cell Invasion Assay utilizes a 3-D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation Extracellular Matrix to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid is embedded in an invasion matrix composed of basement membrane proteins. This matrix forms a hydrogel network on which invasive cells can travel. Invasion modulating agents can be applied to the system to evaluate the impact on cell response. Cell invasion is visualized microscopically and can be quantified through image analysis software.

Kit Contents

• Cultrex Spheroid Formation Matrix
• 3-D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals
• Cultrex Spheroid Invasion Matrix

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.


Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.


For research use only. Not for diagnostic use.

Product Datasheets

Data Example

Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay. Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix. Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix. After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.

Citations for Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. PD-L1 recruits phospholipase C and enhances tumorigenicity of lung tumors harboring mutant forms of EGFR
    Authors: S Ghosh, NB Nataraj, A Noronha, S Patkar, A Sekar, S Mukherjee, S Winograd-K, L Kramarski, A Verma, M Lindzen, DD Garcia, J Green, G Eisenberg, H Gil-Henn, A Basu, Y Lender, S Weiss, M Oren, M Lotem, B Geiger, E Ruppin, Y Yarden
    Cell Reports, 2021;35(8):109181.  2021
  2. Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK
    Authors: I Masi, V Caprara, F Spadaro, L Chellini, R Sestito, A Zancla, A Rainer, A Bagnato, L Rosanò
    Cell Reports, 2021;34(9):108800.  2021
  3. DOCK6 promotes chemo- and radioresistance of gastric cancer by modulating WNT/&beta-catenin signaling and cancer stem cell traits
    Authors: HC Chi, CY Tsai, CS Wang, HY Yang, CH Lo, WJ Wang, KF Lee, LY Lai, JH Hong, YF Chang, MM Tsai, CT Yeh, CH Wu, CC Hsieh, LH Wang, WJ Chen, KH Lin
    Oncogene, 2020;0(0):.  2020
  4. Co-delivery of IKBKE siRNA and cabazitaxel by hybrid nanocomplex inhibits invasiveness and growth of triple-negative breast cancer
    Authors: Z Zhao, Y Li, H Liu, A Jain, PV Patel, K Cheng
    Sci Adv, 2020;6(29):eabb0616.  2020
  5. Integrated pharmaco-proteogenomics defines two subgroups in isocitrate dehydrogenase wild-type glioblastoma with prognostic and therapeutic opportunities
    Authors: S Oh, J Yeom, HJ Cho, JH Kim, SJ Yoon, H Kim, JK Sa, S Ju, H Lee, MJ Oh, W Lee, Y Kwon, H Li, S Choi, JH Han, JH Chang, E Choi, J Kim, NG Her, SH Kim, SG Kang, E Paek, DH Nam, C Lee, HS Kim
    Nat Commun, 2020;11(1):3288.  2020
  6. PAICS, a Purine Nucleotide Metabolic Enzyme, is Involved in Tumor Growth and the Metastasis of Colorectal Cancer
    Authors: S Agarwal, BVSK Chakravart, M Behring, HG Kim, DS Chandrashe, N Gupta, P Bajpai, A Elkholy, SAH Balasubram, C Hardy, SA Diffalha, S Varambally, U Manne
    Cancers (Basel), 2020;12(4):.  2020
  7. Nitidine Chloride Is a Potential Alternative Therapy for Glioma Through Inducing Endoplasmic Reticulum Stress and Alleviating Epithelial-Mesenchymal Transition
    Authors: Z Chen, J Zhang, H Xue, M Qian, X Guo, X Gao, J Xu, Y Qi, X Sun, G Li
    Integr Cancer Ther, 2020;19(0):1534735419900.  2020
  8. Targeting P4HA1 with a Small Molecule Inhibitor in a Colorectal Cancer PDX Model
    Authors: S Agarwal, M Behring, HG Kim, P Bajpai, BVSK Chakravart, N Gupta, A Elkholy, S Al Diffalh, S Varambally, U Manne
    Transl Oncol, 2020;13(4):100754.  2020
  9. Hypoxia-induced lncRNA PDIA3P1 promotes mesenchymal transition via sponging of miR-124-3p in glioma
    Authors: S Wang, Y Qi, X Gao, W Qiu, Q Liu, X Guo, M Qian, Z Chen, Z Zhang, H Wang, J Xu, H Xue, X Guo, P Zhang, R Zhao, G Li
    Cell Death Dis, 2020;11(3):168.  2020
  10. Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-&kappaB activation
    Authors: J Xu, Z Zhang, M Qian, S Wang, W Qiu, Z Chen, Z Sun, Y Xiong, C Wang, X Sun, R Zhao, H Xue, G Li
    J. Exp. Clin. Cancer Res., 2020;39(1):59.  2020
  11. Low-dose zoledronate for the treatment of bone metastasis secondary to prostate cancer
    Authors: E Akoury, P Ahangar, A Nour, J Lapointe, KP Guérard, L Haglund, DH Rosenzweig, MH Weber
    Cancer Cell Int., 2019;19(0):28.  2019
  12. Aspartate &beta-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway
    Authors: K Ogawa, Q Lin, L Li, X Bai, X Chen, H Chen, R Kong, Y Wang, H Zhu, F He, Q Xu, L Liu, M Li, S Zhang, K Nagaoka, R Carlson, H Safran, K Charpentie, B Sun, J Wands, X Dong
    J Hematol Oncol, 2019;12(1):144.  2019
  13. ASPH-notch Axis guided Exosomal delivery of Prometastatic Secretome renders breast Cancer multi-organ metastasis
    Authors: Q Lin, X Chen, F Meng, K Ogawa, M Li, R Song, S Zhang, Z Zhang, X Kong, Q Xu, F He, X Bai, B Sun, MC Hung, L Liu, J Wands, X Dong
    Mol. Cancer, 2019;18(1):156.  2019
  14. Bacopasides I and II Act in Synergy to Inhibit the Growth, Migration and Invasion of Breast Cancer Cell Lines
    Authors: HM Palethorpe, E Smith, Y Tomita, M Nakhjavani, AJ Yool, TJ Price, JP Young, AR Townsend, JE Hardingham
    Molecules, 2019;24(19):.  2019
  15. MicroRNA-214 targets PTK6 to inhibit tumorigenic potential and increase drug sensitivity of prostate cancer cells
    Authors: P Cagle, S Niture, A Srivastava, M Ramalinga, R Aqeel, L Rios-Colon, U Chimeh, S Suy, SP Collins, R Dahiya, D Kumar
    Sci Rep, 2019;9(1):9776.  2019
  16. Isoliquiritin Apioside Suppresses in vitro Invasiveness and Angiogenesis of Cancer Cells and Endothelial Cells
    Authors: A Kim, JY Ma
    Front Pharmacol, 2018;9(0):1455.  2018
  17. Repositioning Bazedoxifene as a novel IL-6/GP130 signaling antagonist for human rhabdomyosarcoma therapy
    Authors: H Xiao, HK Bid, X Chen, X Wu, J Wei, Y Bian, C Zhao, H Li, C Li, J Lin
    PLoS ONE, 2017;12(7):e0180297.  2017
  18. Estradiol promotes breast cancer cell migration via recruitment and activation of neutrophils
    Authors: G Vazquez Ro, A Abrahamsso, LD Jensen, C Dabrosin
    Cancer Immunol Res, 2017;0(0):.  2017
  19. Tumor Cell Invasion Can Be Blocked by Modulators of Collagen Fibril Alignment That Control Assembly of the Extracellular Matrix.
    Authors: Grossman M, Ben-Chetrit N, Zhuravlev A, Afik R, Bassat E, Solomonov I, Yarden Y, Sagi I
    Cancer Res, 2016;76(14):4249-58.  2016
  20. RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs
    Authors: Hiroaki Kajiho
    EMBO Rep, 2016;0(0):.  2016
  21. Musashi-2 (MSI2) supports TGF-beta signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis.
    Authors: Kudinov A, Deneka A, Nikonova A, Beck T, Ahn Y, Liu X, Martinez C, Schultz F, Reynolds S, Yang D, Cai K, Yaghmour K, Baker K, Egleston B, Nicolas E, Chikwem A, Andrianov G, Singh S, Borghaei H, Serebriiskii I, Gibbons D, Kurie J, Golemis E, Boumber Y
    Proc Natl Acad Sci U S A, 2016;113(25):6955-60.  2016
  22. Binding of 14-3-3 reader proteins to phosphorylated DNMT1 facilitates aberrant DNA methylation and gene expression.
    Authors: Esteve P, Zhang G, Ponnaluri V, Deepti K, Chin H, Dai N, Sagum C, Black K, Correa I, Bedford M, Cheng X, Pradhan S
    Nucleic Acids Res, 2015;0(0):.  2015
  23. The Epstein-Barr virus causes epithelial-mesenchymal transition in human corneal epithelial cells via Syk/src and Akt/Erk signaling pathways.
    Authors: Park G, Kim D, Kim Y, Kim S, Lee H, Yang J, Hur D
    Invest Ophthalmol Vis Sci, 2014;55(3):1770-9.  2014
  24. Regulation of extracellular matrix degradation and metastatic spread by IQGAP1 through endothelin-1 receptor signaling in ovarian cancer.
    Authors: Chellini L, Caprara V, Spadaro F, Sestito R, Bagnato A, Rosano L
    Matrix Biol, 0;0(0):.  0
  25. Multimodular biosensors reveal a novel platform for activation of G proteins by growth factor receptors.
    Authors: Midde K, Aznar N, Laederich M, Ma G, Kunkel M, Newton A, Ghosh P
    Proc Natl Acad Sci U S A, 0;112(9):E937-46.  0
  26. The oncogenic triangle of HMGA2, LIN28B and IGF2BP1 antagonizes tumor-suppressive actions of the let-7 family.
    Authors: Busch B, Bley N, Muller S, Glass M, Misiak D, Lederer M, Vetter M, Strauss H, Thomssen C, Huttelmaier S
    Nucleic Acids Res, 0;44(8):3845-64.  0
  27. CXCL16 signaling mediated macrophage effects on tumor invasion of papillary thyroid carcinoma.
    Authors: Cho S, Kim Y, Sun H, Kim Y, Oh B, Yi K, Park D, Park Y
    Endocr Relat Cancer, 0;23(2):113-24.  0
  28. Endophilin A2 Promotes TNBC Cell Invasion and Tumor Metastasis.
    Authors: Baldassarre T, Watt K, Truesdell P, Meens J, Schneider M, Sengupta S, Craig A
    Mol Cancer Res, 0;13(6):1044-55.  0
  29. The GAS6-AXL signaling network is a mesenchymal (Mes) molecular subtype-specific therapeutic target for ovarian cancer.
    Authors: Antony J, Tan T, Kelly Z, Low J, Choolani M, Recchi C, Gabra H, Thiery J, Huang R
    Sci Signal, 0;9(448):ra97.  0
  30. The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors.
    Authors: Lin A, Hu Q, Li C, Xing Z, Ma G, Wang C, Li J, Ye Y, Yao J, Liang K, Wang S, Park P, Marks J, Zhou Y, Zhou J, Hung M, Liang H, Hu Z, Shen H, Hawke D, Han L, Zhou Y, Lin C, Yang L
    Nat Cell Biol, 0;19(3):238-251.  0
  31. The effect of bone morphogenetic protein-2 on osteosarcoma metastasis.
    Authors: Gill J, Connolly P, Roth M, Chung S, Zhang W, Piperdi S, Hoang B, Yang R, Guzik H, Morris J, Gorlick R, Geller D
    PLoS ONE, 0;12(3):e0173322.  0


  1. For Cultrex 3D BME Cell Invasion Assay, 96 well,catalog # 3500-096-K,  do we measure the protein concentration of the invasion matrix (part # 3500-096-03) supplied in this kit?

    • No. We donot measure the protein concentration of the invasion matrix (part # 3500-096-03) supplied in catalog # 3500-096-K.

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Cultrex 3D Culture BME Cell Invasion Assay, 96 well
By Anonymous on 04/02/2018

easy to use, excellent result