Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well

Catalog # Availability Size / Price Qty
3500-096-K
Spheroid Cell Invasion Assay_3500-096-K
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Product Details
Citations (27)
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Reviews (1)

Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well Summary

Provides a more complete and physiologically predictive cancer invasion model.

Why Use Cultrex 3-D Spheroid Basememnt Membrane Extract Cell Invasion Assay, 96-well?

The Cultrex 3-D Culture Spheroid Cell Invasion Assay utilizes a 3-D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation Extracellular Matrix to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid is embedded in an invasion matrix composed of basement membrane proteins. This matrix forms a hydrogel network on which invasive cells can travel. Invasion modulating agents can be applied to the system to evaluate the impact on cell response. Cell invasion is visualized microscopically and can be quantified through image analysis software.

Kit Contents

• Cultrex Spheroid Formation Matrix
• 3-D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals
• Cultrex Spheroid Invasion Matrix

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Mouse

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay. Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix. Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix. After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.

Citations for Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Integrated pharmaco-proteogenomics defines two subgroups in isocitrate dehydrogenase wild-type glioblastoma with prognostic and therapeutic opportunities
    Authors: S Oh, J Yeom, HJ Cho, JH Kim, SJ Yoon, H Kim, JK Sa, S Ju, H Lee, MJ Oh, W Lee, Y Kwon, H Li, S Choi, JH Han, JH Chang, E Choi, J Kim, NG Her, SH Kim, SG Kang, E Paek, DH Nam, C Lee, HS Kim
    Nat Commun, 2020;11(1):3288.  2020
  2. PAICS, a Purine Nucleotide Metabolic Enzyme, is Involved in Tumor Growth and the Metastasis of Colorectal Cancer
    Authors: S Agarwal, BVSK Chakravart, M Behring, HG Kim, DS Chandrashe, N Gupta, P Bajpai, A Elkholy, SAH Balasubram, C Hardy, SA Diffalha, S Varambally, U Manne
    Cancers (Basel), 2020;12(4):.  2020
  3. Nitidine Chloride Is a Potential Alternative Therapy for Glioma Through Inducing Endoplasmic Reticulum Stress and Alleviating Epithelial-Mesenchymal Transition
    Authors: Z Chen, J Zhang, H Xue, M Qian, X Guo, X Gao, J Xu, Y Qi, X Sun, G Li
    Integr Cancer Ther, 2020;19(0):1534735419900.  2020
  4. Targeting P4HA1 with a Small Molecule Inhibitor in a Colorectal Cancer PDX Model
    Authors: S Agarwal, M Behring, HG Kim, P Bajpai, BVSK Chakravart, N Gupta, A Elkholy, S Al Diffalh, S Varambally, U Manne
    Transl Oncol, 2020;13(4):100754.  2020
  5. Hypoxia-induced lncRNA PDIA3P1 promotes mesenchymal transition via sponging of miR-124-3p in glioma
    Authors: S Wang, Y Qi, X Gao, W Qiu, Q Liu, X Guo, M Qian, Z Chen, Z Zhang, H Wang, J Xu, H Xue, X Guo, P Zhang, R Zhao, G Li
    Cell Death Dis, 2020;11(3):168.  2020
  6. Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-&kappaB activation
    Authors: J Xu, Z Zhang, M Qian, S Wang, W Qiu, Z Chen, Z Sun, Y Xiong, C Wang, X Sun, R Zhao, H Xue, G Li
    J. Exp. Clin. Cancer Res., 2020;39(1):59.  2020
  7. Aspartate &beta-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway
    Authors: K Ogawa, Q Lin, L Li, X Bai, X Chen, H Chen, R Kong, Y Wang, H Zhu, F He, Q Xu, L Liu, M Li, S Zhang, K Nagaoka, R Carlson, H Safran, K Charpentie, B Sun, J Wands, X Dong
    J Hematol Oncol, 2019;12(1):144.  2019
  8. ASPH-notch Axis guided Exosomal delivery of Prometastatic Secretome renders breast Cancer multi-organ metastasis
    Authors: Q Lin, X Chen, F Meng, K Ogawa, M Li, R Song, S Zhang, Z Zhang, X Kong, Q Xu, F He, X Bai, B Sun, MC Hung, L Liu, J Wands, X Dong
    Mol. Cancer, 2019;18(1):156.  2019
  9. Bacopasides I and II Act in Synergy to Inhibit the Growth, Migration and Invasion of Breast Cancer Cell Lines
    Authors: HM Palethorpe, E Smith, Y Tomita, M Nakhjavani, AJ Yool, TJ Price, JP Young, AR Townsend, JE Hardingham
    Molecules, 2019;24(19):.  2019
  10. MicroRNA-214 targets PTK6 to inhibit tumorigenic potential and increase drug sensitivity of prostate cancer cells
    Authors: P Cagle, S Niture, A Srivastava, M Ramalinga, R Aqeel, L Rios-Colon, U Chimeh, S Suy, SP Collins, R Dahiya, D Kumar
    Sci Rep, 2019;9(1):9776.  2019
  11. Low-dose zoledronate for the treatment of bone metastasis secondary to prostate cancer
    Authors: E Akoury, P Ahangar, A Nour, J Lapointe, KP Guérard, L Haglund, DH Rosenzweig, MH Weber
    Cancer Cell Int., 2019;19(0):28.  2019
  12. Isoliquiritin Apioside Suppresses in vitro Invasiveness and Angiogenesis of Cancer Cells and Endothelial Cells
    Authors: A Kim, JY Ma
    Front Pharmacol, 2018;9(0):1455.  2018
  13. Estradiol promotes breast cancer cell migration via recruitment and activation of neutrophils
    Authors: G Vazquez Ro, A Abrahamsso, LD Jensen, C Dabrosin
    Cancer Immunol Res, 2017;0(0):.  2017
  14. Repositioning Bazedoxifene as a novel IL-6/GP130 signaling antagonist for human rhabdomyosarcoma therapy
    Authors: H Xiao, HK Bid, X Chen, X Wu, J Wei, Y Bian, C Zhao, H Li, C Li, J Lin
    PLoS ONE, 2017;12(7):e0180297.  2017
  15. Musashi-2 (MSI2) supports TGF-beta signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis.
    Authors: Kudinov A, Deneka A, Nikonova A, Beck T, Ahn Y, Liu X, Martinez C, Schultz F, Reynolds S, Yang D, Cai K, Yaghmour K, Baker K, Egleston B, Nicolas E, Chikwem A, Andrianov G, Singh S, Borghaei H, Serebriiskii I, Gibbons D, Kurie J, Golemis E, Boumber Y
    Proc Natl Acad Sci U S A, 2016;113(25):6955-60.  2016
  16. Tumor Cell Invasion Can Be Blocked by Modulators of Collagen Fibril Alignment That Control Assembly of the Extracellular Matrix.
    Authors: Grossman M, Ben-Chetrit N, Zhuravlev A, Afik R, Bassat E, Solomonov I, Yarden Y, Sagi I
    Cancer Res, 2016;76(14):4249-58.  2016
  17. RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs
    Authors: Hiroaki Kajiho
    EMBO Rep, 2016;0(0):.  2016
  18. Binding of 14-3-3 reader proteins to phosphorylated DNMT1 facilitates aberrant DNA methylation and gene expression.
    Authors: Esteve P, Zhang G, Ponnaluri V, Deepti K, Chin H, Dai N, Sagum C, Black K, Correa I, Bedford M, Cheng X, Pradhan S
    Nucleic Acids Res, 2015;0(0):.  2015
  19. The Epstein-Barr virus causes epithelial-mesenchymal transition in human corneal epithelial cells via Syk/src and Akt/Erk signaling pathways.
    Authors: Park G, Kim D, Kim Y, Kim S, Lee H, Yang J, Hur D
    Invest Ophthalmol Vis Sci, 2014;55(3):1770-9.  2014
  20. Regulation of extracellular matrix degradation and metastatic spread by IQGAP1 through endothelin-1 receptor signaling in ovarian cancer.
    Authors: Chellini L, Caprara V, Spadaro F, Sestito R, Bagnato A, Rosano L
    Matrix Biol, 0;0(0):.  0
  21. The oncogenic triangle of HMGA2, LIN28B and IGF2BP1 antagonizes tumor-suppressive actions of the let-7 family.
    Authors: Busch B, Bley N, Muller S, Glass M, Misiak D, Lederer M, Vetter M, Strauss H, Thomssen C, Huttelmaier S
    Nucleic Acids Res, 0;44(8):3845-64.  0
  22. CXCL16 signaling mediated macrophage effects on tumor invasion of papillary thyroid carcinoma.
    Authors: Cho S, Kim Y, Sun H, Kim Y, Oh B, Yi K, Park D, Park Y
    Endocr Relat Cancer, 0;23(2):113-24.  0
  23. Endophilin A2 Promotes TNBC Cell Invasion and Tumor Metastasis.
    Authors: Baldassarre T, Watt K, Truesdell P, Meens J, Schneider M, Sengupta S, Craig A
    Mol Cancer Res, 0;13(6):1044-55.  0
  24. The GAS6-AXL signaling network is a mesenchymal (Mes) molecular subtype-specific therapeutic target for ovarian cancer.
    Authors: Antony J, Tan T, Kelly Z, Low J, Choolani M, Recchi C, Gabra H, Thiery J, Huang R
    Sci Signal, 0;9(448):ra97.  0
  25. The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors.
    Authors: Lin A, Hu Q, Li C, Xing Z, Ma G, Wang C, Li J, Ye Y, Yao J, Liang K, Wang S, Park P, Marks J, Zhou Y, Zhou J, Hung M, Liang H, Hu Z, Shen H, Hawke D, Han L, Zhou Y, Lin C, Yang L
    Nat Cell Biol, 0;19(3):238-251.  0
  26. The effect of bone morphogenetic protein-2 on osteosarcoma metastasis.
    Authors: Gill J, Connolly P, Roth M, Chung S, Zhang W, Piperdi S, Hoang B, Yang R, Guzik H, Morris J, Gorlick R, Geller D
    PLoS ONE, 0;12(3):e0173322.  0
  27. Multimodular biosensors reveal a novel platform for activation of G proteins by growth factor receptors.
    Authors: Midde K, Aznar N, Laederich M, Ma G, Kunkel M, Newton A, Ghosh P
    Proc Natl Acad Sci U S A, 0;112(9):E937-46.  0

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Cultrex 3D Culture BME Cell Invasion Assay, 96 well
By Anonymous on 04/02/2018
Application:

easy to use, excellent result