Cultrex Spheroid Formation Extracellular Matrix, 10X Summary
Why Use Cultrex Spheroid Formation Extracellular Matrix, 10X?
The Cultrex 10X Spheroid Formation Extracellular Matrix (ECM) is a proprietary mixture of extracellular matrix proteins derived from murine EHS sarcoma and optimized for spheroid formation. The diluted ECM is a fluid in culture that promotes cell-cell adhesion within a low adhesion environment.
For research use only. Not for diagnositic use.
Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay (Catalog # 3500-096-K). Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix (Catalog # 3500-096-01). Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix (Catalog # 3500-096-03). After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.
Citation for Cultrex Spheroid Formation Extracellular Matrix, 10X
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Hypoxia Associated Factor (HAF) mediates neurofibromin ubiquitination and degradation leading to Ras-ERK pathway activation in hypoxia
Authors: Y Song Green, T Sargis, EC Reichert, E Rudasi, D Fuja, E Jonasch, MY Koh
Mol. Cancer Res., 2019;0(0):. 2019
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