Cultrex Spheroid Invasion Extracellular Matrix

Catalog # Availability Size / Price Qty
3500-096-03
Spheroid Cell Invasion Assay_3500-096-K
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Cultrex Spheroid Invasion Extracellular Matrix Summary

Provides a specialized extracellular matrix that is optimized to drive invasion of cells out of the preformed spheroids as a part of the Cultrex 3-D Culture Spheroid Cell Invasion Assay.

Why Use Cultrex Spheroid Invasion Extracellular Matrix?

The Cultrex Spheroid Invasion Matrix is a proprietary extracellular matrix blend comprised of basement membrane extract, derived from murine EHS sarcoma cells, and collagen I, from bovine extensor tendons. This matrix forms a hydrogel network on which invasive cells can travel out of a preformed spheroid. Cell invasion is visualized microscopically and can be quantified through image analysis software.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Endotoxin Level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Performance tested in Cultrex 3-D Spheroid Cell Invasion Assay - supports invasion of cancer cells (MDA-MB-231 human breast cancer cells or HT-1080 human fibrosarcoma) out of a spheroid over a 96 hour period.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Mouse

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay (Catalog # 3500-096-K). Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix (Catalog # 3500-096-01). Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix (Catalog # 3500-096-03). After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.

Reagents Provided

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