14-3-3 Antibody (SR1457) - BSA Free

Novus Biologicals | Catalog # NBP3-21694

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1457 expressed in HEK293

Format

BSA Free
View all 14-3-3 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human 14-3-3 (Uniprot #: P31946, P63104)

Specificity

Detects endogenous levels of 14-3-3

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 Antibody (SR1457) - BSA Free

Immunohistochemistry: 14-3-3 Antibody (SR1457) [NBP3-21694] -

Immunohistochemistry: 14-3-3 Antibody (SR1457) [NBP3-21694] - Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBP3-21694.

Western Blot: 14-3-3 Antibody (SR1457) [NBP3-21694] -

Western Blot: 14-3-3 Antibody (SR1457) [NBP3-21694] - Western blot analysis using NBP3-21694 in Hela lysate.

Applications for 14-3-3 Antibody (SR1457) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50~1:200

Western Blot

1:500-1:1000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3

14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).

Alternate Names

1433

Gene Symbol

YWHAQ

Additional 14-3-3 Products

Product Documents for 14-3-3 Antibody (SR1457) - BSA Free

Certificate of Analysis

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Product Specific Notices for 14-3-3 Antibody (SR1457) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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