Recombinant Human 14-3-3 epsilon Protein

Name: Recombinant Human 14-3-3 epsilon Protein
Brand: Novus Biologicals
SKU: NBC1-18392
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBC1-18392

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Applications

SDS-PAGE

View all 14-3-3 epsilon Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids1-255 of Human 14-3-3 epsilon

Source: E.coli

Amino Acid Sequence: MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV EDENQ

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human 14-3-3 epsilon Protein

SDS-Page: Recombinant Human 14-3-3 epsilon Protein [NBC1-18392] -

SDS-Page: Recombinant Human 14-3-3 epsilon Protein [NBC1-18392] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBC1-18392

Formulation	20 mM Tris-HCl buffer (pH 7.5)
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 epsilon

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human 14-3-3 epsilon was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

1433 epsilon, YWHAE

Gene Symbol

YWHAE

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Product Documents for Recombinant Human 14-3-3 epsilon Protein

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Product Specific Notices for Recombinant Human 14-3-3 epsilon Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.