Catalog Number: 4474
Chemical Name: (3β)-Cholest-5-ene-3,20-diol
Biological Activity
Allosteric activator of the Hedgehog signaling pathway Smoothened (Smo) oncoprotein; binds at a site distinct from the canonical cyclopamine binding site. Activates Hedgehog (Hh) signaling (EC50 ~ 3μM for induction of Hh reporter gene transcription in in NIH 3T3 cells). Induces Smo accumulation in primary cilia; also exhibits osteogenic activity and activates the liver X receptor (LXR).
Technical Data
  • M.Wt:
    402.65
  • Formula:
    C27H46O2
  • Solubility:
    Soluble to 100 mM in ethanol
  • Purity:
    >95%
  • Storage:
    Store at -20°C
  • CAS No:
    516-72-3
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Oxysterols are allosteric activators of the oncoprotein Smoothened.
    Nachtergaele et al.
    Nat.Chem.Biol., 2012;8:211
  2. Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling.
    Nedelcu et al.
    Nat.Chem.Biol., 2013;9:557
  3. Oxysterols are novel activators of the hedgehog signaling pathway in pluripotent mesenchymal cells
    Dwyer et al.
    J.Biol.Chem., 2007;282:8959

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