Cyclopamine

Tocris Bioscience | Catalog # 1623

Inhibitor of Hedgehog (Hh) signaling
Tocris Bioscience

Key Product Details

Description

Inhibitor of Hedgehog (Hh) signaling
View all Hedgehog Signaling Inhibitors »

Product Description

Cyclopamine is an inhibitor of hedgehog (Hh) signaling, likely via direct inhibition of Smoothened, the accessory protein to the putative Hh receptor Patched. Anticancer and teratogenic in vivo. Depletes stem-like cancer cells in glioblastoma and blocks tumor engraftment. Can also be used to induce differentiation of human embryonic stem cells (hESCs) into hormone-expressing endocrine cells.

Product Specifications for Cyclopamine

Molecular Weight

411.63

Formula

C27H41NO2

Storage

Desiccate at -20°C

Purity

≥97% (HPLC)

Chemical Name

(2'R,3S,3'R,3'aS,6'S,6aS,6bS,7'aR,11aS,11bR)-1,2,3,3'a,4,4',5',6,6',6a,6b,7,7',7'a,8,11,11a,11b-Octadecahydro-3',6',10,11b-tetramethylspiro[9H-benzo[a]fluorene-9,2'(3'H)-furo[3,2-b]pyridin]-3-ol

CAS Number

4449-51-8

PubChem ID

442972

InChI Key

QASFUMOKHFSJGL-LAFRSMQTSA-N

SMILES

O[C@H](C2)CC[C@@]1(C)C2=CC[C@]([C@@]([H])3CC[C@@]54O[C@@](C[C@H](C)CN6)([H])[C@]6([H])[C@H]5C)([H])[C@@]([H])1CC3=[C@@]4C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
Ethanol 2.06 5

Preparing Stock Solutions for Cyclopamine

The following data is based on the product molecular weight 411.63.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 48.59 mL 242.94 mL 485.87 mL
0.25 mM 9.72 mL 48.59 mL 97.17 mL
0.5 mM 4.86 mL 24.29 mL 48.59 mL
2.5 mM 0.97 mL 4.86 mL 9.72 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 82 publications citing the usage of this product.

Product Documents for Cyclopamine

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cyclopamine

For research use only

Customer Reviews for Cyclopamine (1)

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  • Modulators of Hh signalling and stress fibres
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Primary OPCs
    Verified Customer | Posted 11/09/2019
    Modulators of Hh signalling and stress fibres. Cyclopamine (Tocris Bioscience, 1623) was dissolved in ethanol and DMSO. The final concentration used for cells was 5 mM of cyclopamine.
    Cyclopamine 1623

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