Catalog Number: 1623
Chemical Name: (2'R,3S,3'R,3'aS,6'S,6aS,6bS,7'aR,11aS,11bR)-1,2,3,3'a,4,4',5',6,6',6a,6b,7,7',7'a,8,11,11a,11b-Octadecahydro-3',6',10,11b-tetramethylspiro[9H-benzo[a]fluorene-9,2'(3'H)-furo[3,2-b]pyridin]-3-ol
Biological Activity
Inhibitor of hedgehog (Hh) signaling, likely via direct inhibition of Smoothened, the accessory protein to the putative Hh receptor Patched. Anticancer and teratogenic in vivo. Depletes stem-like cancer cells in glioblastoma and blocks tumor engraftment. Can also be used to induce differentiation of human embryonic stem cells (hESCs) into hormone-expressing endocrine cells.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 5 mM in ethanol
  • Purity:
  • Storage:
    Desiccate at -20°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Inhibition of hedgehog signaling by direct binding of cyclopamine to smoothened.
    Chen et al.
    Genes Dev., 2002;16:2743
  2. Cyclopamine-mediated hedgehog pathway inhibition depletes stem-like cancer cells in glioblastoma.
    Bar et al.
    Stem Cells, 2007;25:2524
  3. Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells.
    D'Amour et al.
    Nat.Biotechnol., 2006;24:1392
  4. Cancer: a twist in a hedgehog's tale.
    Nature, 2003;425:780

The citations listed below are publications that use Tocris products. Selected citations for Cyclopamine include:

6 Citations: Showing 1 - 6
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  1. Integration of Shh and Fgf signaling in controlling Hox gene expression in cultured limb cells.
    Authors: Rodrigues
    PNAS 2017;114(12):3139
  2. Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation.
    Authors: Yuan Et al.
    Stem Cells Int 2016;7:11024
  3. Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells.
    Authors: Kumar Et al.
    Elife 2015;4
  4. Sonic hedgehog produced by bone marrow-derived mesenchymal stromal cells supports cell survival in myelodysplastic syndrome.
    Authors: Zou Et al.
    Sci Signal 2015;2015:957502
  5. White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
    Authors: Moisan Et al.
    Nat Cell Biol 2015;17:57
  6. GLI activation by atypical protein kinase C Ι/λ regulates the growth of basal cell carcinomas.
    Authors: Atwood Et al.
    Nat Commun 2013;494:484

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