Chemical Name: (2'R,3S,3'R,3'aS,6'S,6aS,6bS,7'aR,11aS,11bR)-1,2,3,3'a,4,4',5',6,6',6a,6b,7,7',7'a,8,11,11a,11b-Octadecahydro-3',6',10,11b-tetramethylspiro[9H-benzo[a]fluorene-9,2'(3'H)-furo[3,2-b]pyridin]-3-ol
Biological ActivityInhibitor of hedgehog (Hh) signaling, likely via direct inhibition of Smoothened, the accessory protein to the putative Hh receptor Patched. Anticancer and teratogenic in vivo. Depletes stem-like cancer cells in glioblastoma and blocks tumor engraftment. Can also be used to induce differentiation of human embryonic stem cells (hESCs) into hormone-expressing endocrine cells.
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Tocris products are intended for laboratory research use only, unless stated otherwise.
Cyclopamine-mediated hedgehog pathway inhibition depletes stem-like cancer cells in glioblastoma.
Bar et al.
Stem Cells, 2007;25:2524
Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells.
D'Amour et al.
Inhibition of hedgehog signaling by direct binding of cyclopamine to smoothened.
Chen et al.
Genes Dev., 2002;16:2743
Cancer: a twist in a hedgehog's tale.
Citations for Cyclopamine
The citations listed below are publications that use Tocris products. Selected citations for Cyclopamine include:
8 Citations: Showing 1 - 8
Drug Screening Identifies Sigma-1-Receptor as a Target for the Therapy of VWM Leukodystrophy.
Authors: Atzmon Et al.
Front Mol Neurosci 2018;11:336
Chemical inhibition reveals differential requirements of signaling pathways in krasV12- and Myc-induced liver tumors in transgenic zebrafish.
Authors: Yan Et al.
Sci Rep 2017;7:45796
Integration of Shh and Fgf signaling in controlling Hox gene expression in cultured limb cells.
Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation.
Authors: Yuan Et al.
Stem Cells Int 2016;7:11024
Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells.
Authors: Kumar Et al.
Sonic hedgehog produced by bone marrow-derived mesenchymal stromal cells supports cell survival in myelodysplastic syndrome.
Authors: Zou Et al.
Sci Signal 2015;2015:957502
White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
Authors: Moisan Et al.
Nat Cell Biol 2015;17:57
GLI activation by atypical protein kinase C Ι/λ regulates the growth of basal cell carcinomas.
Authors: Atwood Et al.
Nat Commun 2013;494:484
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Modulators of Hh signalling and stress fibres. Cyclopamine (Tocris Bioscience, 1623) was dissolved in ethanol and DMSO. The final concentration used for cells was 5 mM of cyclopamine.