Substrate for sulfotransferase assay
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Citations (3)

3'-Phosphoadenosine-5'-phosphosulfate Summary


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The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at -70 °C for up to six months. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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Background: PAPS

3'-Phosphoadenosine-5'-phosphosulfate (PAPS) is the universal sulfur donor for sulfotransferases. It is synthesized from ATP through two steps. In the first step, one ATP molecule is converted to adenosine-5'-phosphosulfate (APS) by ATP sulfurylase and pyrophosphate (PPi) is generated as a byproduct. In the second step, the APS is converted to PAPS by APS kinase and ADP is generated as a byproduct. To drive the reaction forward, the pyrophosphate can be further degraded to phosphate (Pi) by inorganic pyrophosphatase to eliminate feedback inhibition, and the ADP can be converted back to ATP using pyruvate kinase.

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Citations for 3'-Phosphoadenosine-5'-phosphosulfate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Identification of Galeterone and Abiraterone as Inhibitors of Dehydroepiandrosterone Sulfonation Catalyzed by Human Hepatic Cytosol, SULT2A1, SULT2B1b, and SULT1E1
    Authors: CKY Yip, S Bansal, SY Wong, AJ Lau
    Drug Metab. Dispos., 2018-02-07;0(0):.  2018-02-07
  2. Expression, purification and characterization of human cytosolic sulfotransferase (SULT) 1C4
    Authors: AL Guidry, ZE Tibbs, M Runge-Morr, CN Falany
    Horm Mol Biol Clin Investig, 2017-01-01;0(0):.  2017-01-01
  3. Detection of specific glycosaminoglycans and glycan epitopes by in vitro sulfation using recombinant sulfotransferases.
    Authors: Wu ZL, Prather B, Ethen CM
    Glycobiology, 2010-12-17;21(5):625-33.  2010-12-17


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