Catalog Number: 3842
Chemical Name: 4-Amino-1-β-D-ribofuranosyl-1,3,5-triazin-2(1H)-one
Biological Activity
DNA methyltransferase inhibitor. Incorporates into DNA forming covalent adducts with cellular DNMT1, depleting enzyme activity. Induces demethylation and reactivation of silenced genes. Improves the efficiency of reprogramming of stem cells; induces differentiation of mesenchymal stem cells into cardiomyocytes.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 50 mM in water and to 100 mM in DMSO
  • Purity:
  • Storage:
    Store at RT
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. 5-aza-Cytidine is a potent inhibitor of DNA methyltransferase 3a and induces apoptosis in HCT-116 colon cancer cells via Gadd45- and p53-dependent mechanisms.
    Schneider-Stock et al.
    J.Pharmacol.Exp.Ther., 2005;312:525
  2. Dissecting direct reprogramming through integrative genomic analysis.
    Mikkelsen et al.
    Nature, 2008;454:49
  3. 5-Azacytidine induces cardiac differentiation of human umbilical cord-derived mesenchymal stem cells by activating extracellular regulated kinase.
    Qian et al.
    Stem Cells Dev., 2012;21:67

The citations listed below are publications that use Tocris products. Selected citations for 5-Azacytidine include:

4 Citations: Showing 1 - 4
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  1. PDGF-AB and 5-Azacytidine induce conversion of somatic cells into tissue-regenerative multipotent stem cells
    Authors: Chandrakanthan Et al.
    Proc Natl Acad Sci U S A 2016;113:E2306
  2. 5-azacytidine inhibits nonsense-mediated decay in a MYC-dependent fashion.
    Authors: Bhuvanagiri Et al.
    EMBO Mol Med 2014;6:1593
  3. Progenitor cells identified by PDGFR-α expression in the developing and diseased human heart.
    Authors: Chong Et al.
    Cell Res 2013;22:1932
  4. Relation of DNA methylation of 5'-CpG island of ACSL3 to transplacental exposure to airborne polycyclic aromatic hydrocarbons and childhood asthma.
    Authors: Perera Et al.
    PLoS One 2009;4:e4488

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