5-BrdU
Chemical Name: 5-Bromo-2-deoxyuridine
Purity: ≥99%
Biological Activity
Synthetic thymidine analog; incorporated into DNA during replication. Used in assays for cell proliferation. Replaces Oct-4 in transcription factor-mediated reprogramming of somatic cells and can be used in small molecule cocktail to generate ciPSCs.Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Cell cycle studies basd upon quantitative image analysis.
Stacey and Hitomi
Cytometry A, 2008;73A:270 -
A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.
Barker et al.
PLoS One, 2013;8:e63692 -
Bromodeoxyuridine promotes full-chemical induction of mouse pluripotent stem cells.
Long et al.
Cell Res., 2015;25:1171
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I used BrdU to monitor cell proliferation in the mouse brain. Preparation of stock solutions of the drug is performed with warm (50ºC) sterile saline. It is advisable to keep the solution for 1h in the water bath at 50ºC with sonicator turned ON to complete solubilization. Aliquots can be kept at -80ºC until the use. The drug was diluted in saline 0.9% and injected i.p. (100 mg/kg). Intracardial perfusion was done 4 h later and BrdU was immunolabeled with an anti-BrdU antibody. The image displays that BrdU is present in the nucleus of the newly formed cells.
