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5-BrdU

5015 1 Review Datasheet / COA / SDS
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5-BrdU | CAS No. 59-14-3 | Buffers, Chelators and Reagents
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Description: Synthetic thymidine analog; used for cell proliferation assays

Chemical Name: 5-Bromo-2-deoxyuridine

Purity: ≥99%

Product Details
Reviews (1)
Biological Activity Technical Data Background Product Datasheets Calculators

Biological Activity

Synthetic thymidine analog; incorporated into DNA during replication. Used in assays for cell proliferation. Replaces Oct-4 in transcription factor-mediated reprogramming of somatic cells and can be used in small molecule cocktail to generate ciPSCs.

Technical Data

M.Wt:
307.1
Formula:
C9H11BrN2O5
Solubility:
Soluble to 50 mM in water and to 100 mM in DMSO
Purity:
≥99%
Storage:
Store at -20°C
CAS No:
59-14-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Proliferation marker for studying neurogenesis
By Luiz Alexandre Magno on 07/17/2018
Application: Species: Mouse

I used BrdU to monitor cell proliferation in the mouse brain. Preparation of stock solutions of the drug is performed with warm (50ºC) sterile saline. It is advisable to keep the solution for 1h in the water bath at 50ºC with sonicator turned ON to complete solubilization. Aliquots can be kept at -80ºC until the use. The drug was diluted in saline 0.9% and injected i.p. (100 mg/kg). Intracardial perfusion was done 4 h later and BrdU was immunolabeled with an anti-BrdU antibody. The image displays that BrdU is present in the nucleus of the newly formed cells.

PMID: 17020783
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