5-BrdU

Catalog # Availability Size / Price Qty
5015/50
Cat.No. 5015 - 5-BrdU | C9H11BrN2O5 | CAS No. 59-14-3
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Description: Synthetic thymidine analog; used for cell proliferation assays

Chemical Name: 5-Bromo-2-deoxyuridine

Product Details
Reviews (1)

Biological Activity

Synthetic thymidine analog; incorporated into DNA during replication. Used in assays for cell proliferation.

Technical Data

Formula:
C9H11BrN2O5
Solubility:
Soluble to 50 mM in water and to 100 mM in DMSO
Storage:
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
CAS No:
59-14-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.

Product Datasheets

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Proliferation marker for studying neurogenesis
By Luiz Alexandre Magno on 07/17/2018
Species: Mouse

I used BrdU to monitor cell proliferation in the mouse brain. Preparation of stock solutions of the drug is performed with warm (50ºC) sterile saline. It is advisable to keep the solution for 1h in the water bath at 50ºC with sonicator turned ON to complete solubilization. Aliquots can be kept at -80ºC until the use. The drug was diluted in saline 0.9% and injected i.p. (100 mg/kg). Intracardial perfusion was done 4 h later and BrdU was immunolabeled with an anti-BrdU antibody. The image displays that BrdU is present in the nucleus of the newly formed cells.

PMID: 17020783

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