5-BrdU

Tocris Bioscience | Catalog # 5015

Synthetic thymidine analog; used for cell proliferation assays
Tocris Bioscience

Key Product Details

Description

Synthetic thymidine analog; used for cell proliferation assays
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Product Description

5-BrdU is a synthetic thymidine analog; incorporated into DNA during replication. Used in assays for cell proliferation. Replaces Oct-4 in transcription factor-mediated reprogramming of somatic cells and can be used in small molecule cocktail to generate ciPSCs.

Product Specifications for 5-BrdU

Molecular Weight

307.10

Formula

C9H11BrN2O5

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

5-Bromo-2-deoxyuridine

CAS Number

59-14-3

PubChem ID

6035

InChI Key

WOVKYSAHUYNSMH-RRKCRQDMSA-N

SMILES

O[C@H]1C[C@H](N2C=C(Br)C(NC2=O)=O)O[C@@H]1CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 15.36 50
DMSO 30.71 100

Preparing Stock Solutions for 5-BrdU

The following data is based on the product molecular weight 307.10.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.26 mL 16.28 mL 32.56 mL
5 mM 0.65 mL 3.26 mL 6.51 mL
10 mM 0.33 mL 1.63 mL 3.26 mL
50 mM 0.07 mL 0.33 mL 0.65 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.

Product Documents for 5-BrdU

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 5-BrdU

For research use only

Customer Reviews for 5-BrdU (1)

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  • Proliferation marker for studying neurogenesis
    Name: Luiz Alexandre Magno
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: Brain
    Verified Customer | Posted 07/17/2018
    I used BrdU to monitor cell proliferation in the mouse brain. Preparation of stock solutions of the drug is performed with warm (50ºC) sterile saline. It is advisable to keep the solution for 1h in the water bath at 50ºC with sonicator turned ON to complete solubilization. Aliquots can be kept at -80ºC until the use. The drug was diluted in saline 0.9% and injected i.p. (100 mg/kg). Intracardial perfusion was done 4 h later and BrdU was immunolabeled with an anti-BrdU antibody. The image displays that BrdU is present in the nucleus of the newly formed cells.
    5-BrdU 5015

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