5-MethylCytosine ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP2-62131
Discontinued Product
NBP2-62131 has been discontinued.
View all 5-MethylCytosine products.
Key Product Details
Sample Type & Volume Required Per Well
DNA samples (100 uL)
Sensitivity
~0.5% 5-methylcytosine per 100ng single-stranded DNA
Assay Range
5-100% (100 ng/ul
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Product Specifications
Assay Type
Indirect ELISA
Kit Type
ELISA Kit (Colorimetric)
Scientific Data Images for 5-MethylCytosine ELISA Kit (Colorimetric)
ELISA: 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131]
ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - The workflow for the 5-Methylcytosine DNA ELISA kit utilizes the indirect ELISA methodology where denatured, single-stranded DNA (ssDNA) samples are coated on the plate well surfaces and a 5-mC mAb and conjugate HRP-Ab are added to the wells. Indirect detELISA: 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131]
ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - Comparison of genomic DNA Mass Spectroscopy versus 5-MethylCytosine ELISA analysis. Genomic DNA samples include: human brain (HB), human kidney (HK), human embryonic stem cell (HESC), mouse brain (MB), mouse kidney (MK), and mouse testes (MT).ELISA: 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131]
ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - A typical standard curve for 5-MethylCytosine ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.Kit Contents for 5-MethylCytosine ELISA Kit (Colorimetric)
- Antibody-conjugate
- Coating Buffer
- Controls
- ELISA Buffer
- HRP Developer
- Microtiter Plate
- Primary Antibody
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: 5-MethylCytosine
Alternate Names
5-mC, 5-Methyl Cytosine, Cytosine (5-Methyl)
Additional 5-MethylCytosine Products
Product Documents for 5-MethylCytosine ELISA Kit (Colorimetric)
Certificate of Analysis
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Product Specific Notices for 5-MethylCytosine ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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