5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-24452

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and animal tissue

Sensitivity

28.0 U/L

Assay Range

28.0 - 581 U/L
View all 5'-Nucleotidase/CD73 Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Species

Non-species specific

Description

Detection instrument: Microplate reader (550 nm)
Assay time: 30 min

Precision

Intra-Assay Precision (Precision within an assay) %CV 3.3

Inter-Assay Precision (Precision between assays) %CV 6

Recovery for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 105%

Scientific Data Images for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] -

5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] - Standard Reference Curve
5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] -

5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] - Assay Example

Kit Contents for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Working Solution
  • Reagent 2: Chromogenic Agent
  • Reagent 3: 0.6 mmol/L Inosine Standard Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 5'-Nucleotidase/CD73

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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FAQs for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

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    • Q: We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
      A: We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
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