ABCA9 Antibody

Novus Biologicals | Catalog # NB100-93469

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ABCA9 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QRVVQELEMENIQD corresponding to internal region according to NP_525022.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

184 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABCA9 Antibody

Immunohistochemistry-Paraffin: ABCA9 Antibody [NB100-93469]

Immunohistochemistry-Paraffin: ABCA9 Antibody [NB100-93469]

Immunohistochemistry-Paraffin: ABCA9 Antibody [NB100-93469] - Staining of paraffin embedded Human Testis with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: ABCA9 Antibody [NB100-93469]

Flow Cytometry: ABCA9 Antibody [NB100-93469]

Flow Cytometry: ABCA9 Antibody [NB100-93469] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for ABCA9 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000
Application Notes
WB: Approx. 170 kDa band observed in human brain (cerebellum) lysates (calculated MW of 184 kDa band according to NP_525022.2).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABCA9

This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two transmembrane domains and two nucleotide binding folds. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This gene is a member of the ABC1 subfamily and is clustered with four other ABC1 family members on chromosome 17q24. Transcriptional expression of this gene is induced during monocyte differentiation into macrophages and is suppressed by cholesterol import. [provided by RefSeq]

Alternate Names

ATP-binding cassette A9, ATP-binding cassette sub-family A member 9, ATP-binding cassette, sub-family A (ABC1), member 9, DKFZp686F2450, EC 3.6.3, EC 3.6.3.41, EST640918, MGC75415

Entrez Gene IDs

10350 (Human)

Gene Symbol

ABCA9

UniProt

Q8IUA7 (Human)

Additional ABCA9 Products

Product Documents for ABCA9 Antibody

Certificate of Analysis

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Product Specific Notices for ABCA9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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