Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ABRO Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human ABRO fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ABRO Antibody - BSA Free

Western Blot: ABRO Antibody [NBP2-97533]

Western Blot: ABRO Antibody [NBP2-97533]

Western Blot: ABRO Antibody [NBP2-97533] - Lane A: U-251 MG Whole Cell LysateLane B: NIH-3T3 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:47 kDaObserved band size:50 kDa
ABRO Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ABRO Antibody [NBP2-97533] -

Immunocytochemistry/Immunofluorescence: ABRO Antibody [NBP2-97533] - Immunofluorescence staining of ABRO in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97533 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for ABRO Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABRO

ABRO, also known as KIAA0157, is a hypothetical protein predicted from the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. The function of ABRO has not been characterized.

Alternate Names

Abraxas brother protein 1, ABRO1abraxas brother 1, Em:AC068896.4, family with sequence similarity 175, member B, FLJ22338, KIAA0157BRISC complex subunit Abro1, Protein FAM175B

Gene Symbol

ABRAXAS2

Additional ABRO Products

Product Documents for ABRO Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ABRO Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ABRO Antibody - BSA Free

There are currently no reviews for this product. Be the first to review ABRO Antibody - BSA Free and earn rewards!

Have you used ABRO Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies