AbSelect (TM) Antibody Concentration and Clean Up Antibody Purification Kit

Novus Biologicals | Catalog # 861-0010

Novus Biologicals
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Key Product Details

Kit Type

Antibody Purification Kit

Product Summary

Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores.

FeaturesBenefits
Quickly and easily concentrate antibodies and proteinsEasy and quick to use
Remove non-protein additives such as sodium azide, tris and glycineHigh recoveries, around 60 - 100%
Perform a simple buffer exchange for more favourable conjugation conditionsSuitable for Lightning-Link kits and for other kits


The antibody concentration and clean-up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution.

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C.

Background: AbSelect (TM) Antibody Concentration and Clean Up

The Antibody concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris. The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.

Additional AbSelect (TM) Antibody Concentration and Clean Up Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations for AbSelect (TM) Antibody Concentration and Clean Up Antibody Purification Kit

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Ascites fluid from mouse (1 ml)
    Species: Mouse
    Verified Customer | Posted 05/15/2014
    Kit components: 5 different buffers and a purification column
    AbSelect (TM) Antibody Concentration and Clean Up Antibody Purification Kit 861-0010

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FAQs

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  • Q: Is the NB500-169 still available in the concentration of 2mg/ml? We ordered five vials in the past month with the unit size 0.1 mg. Three of them arrived with a concentration of 2mg/ml, the last two vial with a concentration of 1mg/ml. Can we influence the concentration when ordering?

    A: In terms of the concentration of the vials, unfortunately sometimes the concentrations of our products may change. (The concentration of each vial is that stated on the label.) I am sorry, but it is not possible for you to order specifically 2 mg/ml vials of NB500-169. (If you require a concentration of 2 mg/ml, you may be interested in our Antibody Concentration and Clean Up Kit (product code for 1 spin column is 861-0005; 3 spin columns is 861-0010), which you could use to reduce the volume of the antibody by half and hence double the concentration.)

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