Catalog # Availability Size / Price Qty
Ac-Cys-NHMe | CAS No. 10061-65-1 | Unnatural Amino Acids
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Description: Building block for the lysine acylation using conjugating enzymes (LACE) technique

Chemical Name: (2R)-2-(Acetylamino)-3-mercapto-N-methylpropanamide

Product Details

Biological Activity

Ac-Cys-NHMe is a non-canonical amino acid that is an essential building block for lysine acylation using conjugating enzymes (LACE) technique. LACE is a chemoenzymatic approach that uses an E2 small ubiquitin-like modifier (SUMO)-conjugating enzyme, Ubc9, to conjugate peptide thioesters containing a C-terminal ubiquitin-derived sequence (LRLRGG) to a lysine residue located in a recognition sequence (IKQE) on a protein via an isopeptide bond. Ac-Cys-NHMe can be reacted with a peptide hydrazide to form a reactive peptide thioester, which is the substrate for Ubc9. LACE enables the site-specific modification of proteins at lysine residues to allow attachment of biochemical probes such as fluorophores to proteins or to engineer proteins for site-specific delivery.

Technical Data

Soluble to 100 mM in DMSO and to 100 mM in water
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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