Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC)

R&D Systems | Catalog # S-330

R&D Systems
Discontinued Product
S-330 has been discontinued. View all Proteasome Substrates products.

Key Product Details

Source

Synthetic

Conjugate

7-Amino-4-Methyl Coumarin
View all Proteasome Substrates Small Molecules and Peptides »

Formulation, Preparation, and Storage

S-330
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Proteasome Substrates

The 20S Proteasome is the catalytic core component of the multi-complex 26S Proteasome that selectively degrades intracellular proteins. It is commonly associated with regulatory complexes, which include the 19S Proteasome, the PA28 alpha/beta complex, or the PA28 gamma complex. The 20S Proteasome is composed of 28 subunits arranged into four stacked rings. The outer rings, containing seven subunits each, are composed of closely-related but non-identical alpha subunits. The amino-terminal tails of the alpha subunits form a gate that restricts substrate entry into the catalytic core. The inner rings, also containing seven subunits each, are composed of closely-related but non-identical beta subunits. The amino-terminal tails of six of the beta subunits, three per ring, have proteolytic activity. Inhibition of 20S Proteasome proteolytic core activity using small molecule inhibitors is a valuable tool for the functional study of a variety of proteins and for therapeutic intervention. The 20S Proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28 Activator alpha.This fluorogenic tri-peptide substrate may be used to monitor Proteasome activity. It is hydrolyzed by the chymotrypsin-like activity of the beta 5c (PSMB5) subunit.

Additional Proteasome Substrates Products

Product Documents for Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC)

Certificate of Analysis

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Product Specific Notices for Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC)

For research use only

Related Research Areas

Notch Pathway

Citations for Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC)

Customer Reviews for Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC) (1)

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  • Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC)
    Name: TAKASHI SHIMADA
    Verified Customer | Posted 05/02/2020
    S-330 hydrolysis confirmation by LCMS-SRM S-330: 1 uM i20S: 15 nM PA28a: 150 nM Reaction: 37 °C for 5 h 1:S-330 Ac-WLA-AMC (588.20>342.15) 2:Free AMC (176.25>120.00) 3:S-330 Ac-WLA-COOH (431.25>342.15)
    Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC) S-330

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