Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC)

Catalog # Availability Size / Price Qty
S-330-02M
Product Details
Citations (2)
FAQs
Reviews (1)

Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC) Summary

The 20S Proteasome is the catalytic core component of the multi-complex 26S Proteasome that selectively degrades intracellular proteins. It is commonly associated with regulatory complexes, which include the 19S Proteasome, the PA28 alpha/beta complex, or the PA28 gamma complex. The 20S Proteasome is composed of 28 subunits arranged into four stacked rings. The outer rings, containing seven subunits each, are composed of closely-related but non-identical alpha subunits. The amino-terminal tails of the alpha subunits form a gate that restricts substrate entry into the catalytic core. The inner rings, also containing seven subunits each, are composed of closely-related but non-identical beta subunits. The amino-terminal tails of six of the beta subunits, three per ring, have proteolytic activity. Inhibition of 20S Proteasome proteolytic core activity using small molecule inhibitors is a valuable tool for the functional study of a variety of proteins and for therapeutic intervention. The 20S Proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28 Activator alpha.This fluorogenic tri-peptide substrate may be used to monitor Proteasome activity. It is hydrolyzed by the chymotrypsin-like activity of the beta 5c (PSMB5) subunit.

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-Species

Product Datasheets

Reagents Provided

Citations for Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth
    Authors: MJ Lee, Z Miller, JE Park, D Bhattarai, W Lee, KB Kim
    Sci Rep, 2019;9(1):4089.  2019
  2. Immunoproteasome functions explained by divergence in cleavage specificity and regulation
    Authors: MB Winter, F La Greca, S Arastu-Kap, F Caiazza, P Cimermanci, TJ Buchholz, JL Anderl, M Ravalin, MF Bohn, A Sali, AJ O'Donoghue, CS Craik
    Elife, 2017;6(0):.  2017

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Reviews for Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC)

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Ac-Trp-Leu-Ala-AMC (Ac-WLA-AMC)
By Anonymous on 05/02/2020
Application:

S-330 hydrolysis confirmation by LCMS-SRM
S-330: 1 uM
i20S: 15 nM
PA28a: 150 nM
Reaction: 37 °C for 5 h
1:S-330 Ac-WLA-AMC (588.20>342.15)
2:Free AMC (176.25>120.00)
3:S-330 Ac-WLA-COOH (431.25>342.15)