Ac₄ManNDAz

Key Product Details

Product Description

Ac₄ManNDAz is a cell-permeable photo-crosslinking probe for studying glycoprotein or glycolipid interactions. The compound is an unnatural diazirine-containing monosaccharide building block, which acts as a photo-crosslinker. Once inside the cell, Ac₄ManNDAz is metabolized and incorporated into cell surface glycoproteins as a photo-crosslinking sialic acid analog. Upon UV irradiation, the diazirine group is activated to a reactive carbene that covalently crosslinks to nearby molecules.

Product Specifications for Ac₄ManNDAz

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for Ac₄ManNDAz

The following data is based on the product molecular weight 457.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.19 mL	10.93 mL	21.86 mL
5 mM	0.44 mL	2.19 mL	4.37 mL
10 mM	0.22 mL	1.09 mL	2.19 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Cervin GM1 ganglioside-independent intoxication by Cholera toxin. PLoS.Pathog. 2018 PMID: 29432456
• Bond Photocrosslinking of glycoconjugates using metabolically incorporated diazirine-containing sugars. Nat.Protoc. 2009 PMID: 19536272
• Bond Metabolism of diazirine-modified N-acetylmannosamine analogues to photo-cross-linking sialosides. Bioconjug.Chem. 2011 PMID: 21838313
• Tanaka Photoactivatable crosslinking sugars for capturing glycoprotein interactions. J.Am.Chem.Soc. 2008 PMID: 18293988

Product Documents for Ac₄ManNDAz