Acridine Orange hydrochloride

Tocris Bioscience | Catalog # 5092

Nucleic acid binding dye. Cell permeable. Used for: cell cycle and apoptosis determination. Application: flow cytometry, fluorescence microscopy
Tocris Bioscience

Key Product Details

Description

Nucleic acid binding dye. Cell permeable. Used for: cell cycle and apoptosis determination. Application: flow cytometry, fluorescence microscopy
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Product Description

Key information: Acridine Orange hydrochloride is a nucleic acid binding dye. Cell and organelle permeable.

Used for: cell cycle and apoptosis determination, for visual detection of nucleic acids on agarose and polyacrylamide gels, for autophagy analyze.

Application: suitable for flow cytometry and fluorescence microscopy.

Properties and Photophysical Data: emits green fluorescence when incorporated into double-stranded DNA, red fluorescence when bound electrostatically to phosphate groups of RNA or single-stranded DNA, and orange fluorescence in acidic conditions. Excitation and emission maxima (λ) are 502 nm and 525 nm, respectively (green, double strands)/ 460 nm and 650 nm, respectively (red, single strands)/ and 475 nm and 590 nm, respectively (orange, acidic conditions).

This product is supplied as a hydrochloride salt, the stoichiometry of which is batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

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Product Specifications for Acridine Orange hydrochloride

Formula

C17H19N3.HCl

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride

PubChem ID

517204

InChI Key

VSTHNGLPHBTRMB-UHFFFAOYSA-N

SMILES

CN(C)C1=CC=C2C(N=C(C=C(N(C)C)C=C3)C3=C2)=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble to 10mg/ml in water

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Product Documents for Acridine Orange hydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Acridine Orange hydrochloride

For research use only

Citations for Acridine Orange hydrochloride

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