Acridonylalanine
Tocris Bioscience | Catalog # 8086
Key Product Details
Description
Wavelength
Product Description
Key information: Acridonylalanine is a fluorescent unnatural amino acid. Can be used to specifically label a protein of interest during protein translation. Suitable for lifetime and fluorescence resonance energy transfer (FRET) studies.Used for: labeling proteins, monitoring protein interactions and conformational changes through fluorescence polarization or lifetime experiments.
Application: fluorescence lifetime imaging microscopy (FLIM), FRET interactions with common fluorophores such as methoxycoumarin.
Properties and Photophysical Data: highly photostable, high quantum yield and long fluorescence lifetime in water. Excitation and emission maxima (λ) are 385 nm and 420 nm, respectively.
Scientific Data Images for Acridonylalanine
Source material comparison.
Left three bars show the fluorescence intensity of Acridonylalanine (Acd) in GFP protein (incorporated by genetic code expansion at position 151). The source of Acd material originates from either the laboratories of Professor E. James Peterson (EJP) or of Tocris / Bio-Techne (BT). Results are an average of 3 protein expressions in E. coli. The protein without Acd incorporation is included as a negative control. Right three bars: WT GFP expression to determine whether there are any growth effects from Acd in the presence of EJP or BT synthesized Acd.Optical Data
| λabs | 385 nm |
| λem | 420 nm |
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Product Specifications for Acridonylalanine
Molecular Weight
Formula
Storage
Purity
Chemical Name
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 3.04 | 10 | |
| DMSO | 3.04 | 10 |
Preparing Stock Solutions for Acridonylalanine
The following data is based on the product molecular weight 304.28.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 32.86 mL | 164.32 mL | 328.64 mL |
| 0.5 mM | 6.57 mL | 32.86 mL | 65.73 mL |
| 1 mM | 3.29 mL | 16.43 mL | 32.86 mL |
| 5 mM | 0.66 mL | 3.29 mL | 6.57 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Speight Efficient synthesis and in vivo incorporation of acridon-2-ylalanine, a fluorescent amino acid for lifetime and Förster resonance energy transfer/luminescence resonance energy transfer studies. J.Am.Chem.Soc. 2013 PMID: 24303933
- Hostetler Systematic evaluation of soluble protein expression using a fluorescent unnatural amino acid reveals no reliable predictors of tolerability. ACS Chem.Biol. 2018 PMID: 30216041
Product Documents for Acridonylalanine
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Acridonylalanine
For research use only
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