ACSL5 Antibody (CL0275) - BSA Free

Novus Biologicals | Catalog # NBP2-52899

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0275

Format

BSA Free
View all ACSL5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9ULC5, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ACSL5 Antibody (CL0275) - BSA Free

Western Blot: ACSL5 Antibody (CL0275) [NBP2-52899]

Western Blot: ACSL5 Antibody (CL0275) [NBP2-52899]

Western Blot: ACSL5 Antibody (CL0275) [NBP2-52899] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: ACSL5 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899]

Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899]

Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human small intestine shows very strong cytoplasmic and membrane immunoreactivity in the glandular cells.
Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899]

Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899]

Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human colon (colorectal cancer) shows moderate membrane positivity in cancer cells.
Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899]

Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899]

Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human colon shows strong positivity in the intestinal glands.
Immunohistochemistry: ACSL5 Antibody (CL0275) [NBP2-52899]

Immunohistochemistry: ACSL5 Antibody (CL0275) [NBP2-52899]

Immunohistochemistry: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human duodenum shows strong cytoplasmic and membrane immunoreactivity in the glandular cells.
Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899]

Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899]

Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human liver shows moderate cytoplasmic immunoreactivity in hepatocytes.

Applications for ACSL5 Antibody (CL0275) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACSL5

Long-chain-fatty-acid--CoA ligase 5, or ACSL5, is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. These proteins play an important role in both synthesis of cellular lipids and degradation via beta-oxidation. Like other isozymes of this family, ALCS5 converts free long-chain fatty acids into fatty acyl-CoA esters. Specifically, ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage.

This isozyme is highly expressed in uterus and spleen, and in trace amounts are found in normal brain. It also has significantly increased expression in malignant gliomas, where the protein functions in mediating fatty acid-induced glioma cell growth. Therefore, ALCS5 is thought to have a key role in the survival of glioma cells and may be a potential target for novel cancer treatments. Three transcript variants encoding two different isoforms have been found for this gene.

Alternate Names

ACS2, ACS5FACL5 for fatty acid coenzyme A ligase 5, acyl-CoA synthetase long-chain family member 5, EC 6.2.1, EC 6.2.1.3, FACL5fatty acid coenzyme A ligase 5, fatty-acid-Coenzyme A ligase, long-chain 5, LACS 5, Long-chain acyl-CoA synthetase 5, long-chain fatty acid coenzyme A ligase 5, long-chain-fatty-acid--CoA ligase 5

Gene Symbol

ACSL5

Additional ACSL5 Products

Product Documents for ACSL5 Antibody (CL0275) - BSA Free

Certificate of Analysis

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Product Specific Notices for ACSL5 Antibody (CL0275) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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