Adenovirus 2 E1A Antibody (M73) - BSA Free

Novus Biologicals | Catalog # NBP2-66587

Novus Biologicals
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Key Product Details

Species Reactivity

Adenovirus

Applications

Immunoaffinity Purification

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # M73

Format

BSA Free
View all Adenovirus 2 E1A Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant adenovirus 2 E1A protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Adenovirus 2 E1A Antibody (M73) - BSA Free

Application
Recommended Usage

Immunoaffinity Purification

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Adenovirus 2 E1A

The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A protein has both transforming and trans-activating activities. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Disrupts the function of host retinoblastoma protein RB1/pRb and isoform early E1A 26 kDa protein stabilizes TP53, which are key regulators of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif.

Alternate Names

Early E1A protein

Additional Adenovirus 2 E1A Products

Product Documents for Adenovirus 2 E1A Antibody (M73) - BSA Free

Certificate of Analysis

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Product Specific Notices for Adenovirus 2 E1A Antibody (M73) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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