AE2 Antibody - BSA Free

Western Blot: AE2 Antibody [NBP1-59858]

Western Blot: AE2 Antibody [NBP1-59858]

Western Blot: AE2 Antibody - BSA Free [NBP1-59858] -

Inhibition of induction of carbonic anhydrase II (CAII), anion exchange protein 2 (Ae2), chloride channel 7 (ClC-7), vacuolar-type H(+)-ATPase (V-ATPase), and cathapsin K (A,B), matrix metalloproteinase 9 (MMP-9) secretion (C), and bone resorption (D) by aesculetin. Raw 264.7 cells were cultured in minimum essential medium alpha medium ( alpha -MEM) with 50 ng/mL receptor activator of nuclear factor-kappa B ligand (RANKL) in the absence or presence of 1–10 μM aesculetin for 5 days. Whole-cell lysates were subject to SDS-PAGE and Western blot with a specific antibody against CAII, Ae2, ClC-7 V-ATPase, and cathapsin K (A,B). An equal volume of culture medium was subject to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot with a specific antibody against MMP-9 (C). beta -Actin was used as internal control. The bar graphs (mean +/- SEM, n = 3) represent quantitative results of blots obtained from a densitometer. Respective values in double-bar graphs not sharing a small letter are significantly different at p < 0.05. The osteoclast bone resorption was assayed using a commercially available bone resorption assay kit ((D), three separate experiments). Attached cells were removed, and resorption pits on the plate were visualized under light microscopy. Scale bar = 20 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33203061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.