Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AFF4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 50 and 100 of human major CDK9 elongation factor-associated protein of human using the numbering given in entry NP_055238.1 (GeneID 27125).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AFF4 Antibody - BSA Free

Immunoprecipitation: AFF4 Antibody [NBP1-40356]

Immunoprecipitation: AFF4 Antibody [NBP1-40356]

Immunoprecipitation: AFF4 Antibody [NBP1-40356] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-MCEF antibody used for IP at 10 mcg/mg lysate. MCEF was also immunoprecipitated by rabbit anti-MCEF antibody NBP1-40357 which recognizes a downstream epitope.

Applications for AFF4 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

5-15 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AFF4

AFF4, or AF4/FMR2 family member 4, contains a 127 kDa, 98 kDa, and 39 kDa isoform, and is involved in the process of improving the catalytic rate of RNA polymerase II transcription. Disease research is currently being conducted with AFF4 on its relation to acute lymphoblastic leukemia, as well as other types of leukemia. This protein interacts with SIAH1, CCNT1, MLLT3, MLLT1, and MED10 during the regulation of DNA-dependent transcription and the transcription of RNA polymerase II.

Alternate Names

AF4/FMR2 family, member 4, AF5Q31AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, Major CDK9 elongation factor-associated protein, MCEFALL1 fused gene from 5q31, MGC75036, Protein AF-5q31

Entrez Gene IDs

27125 (Human)

Gene Symbol

AFF4

Additional AFF4 Products

Product Documents for AFF4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for AFF4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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