Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 036 expressed in HEK293

Format

Azide and BSA Free
View all Ago2/eIF2C2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Ago2/eIF2C2 (Uniprot#: Q8CJG0; Met1-Ala860).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Ago2/eIF2C2 Antibody (036) - Azide and BSA Free

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - Immunochemical staining of AGO2 in human brain with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - Immunochemical staining of AGO2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - AGO2 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-Mouse AGO2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse AGO2 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size: 100 kDa
Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675]

Immunoprecipitation: Ago2/eIF2C2 Antibody (036) [NBP2-90675] - AGO2 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate. Lane B:0.5 mg MCF-7 Whole Cell Lysate. Lane C:0.5 mg HEK293 Whole Cell Lysate 4 uL anti-AGO2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-AGO2 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size :100 kDa.

Applications for Ago2/eIF2C2 Antibody (036) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1-4 uL/mg of lysate

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ago2/eIF2C2

Ago2 / eIF2C2 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Alternate Names

AGO2argonaute 2, Argonaute2, EC 3.1.26.n1, EC 3.1.26.n2, eIF2C 2, eIF-2C 2, Eukaryotic translation initiation factor 2C 2, eukaryotic translation initiation factor 2C, 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, protein argonaute-2, Protein slicer, Q10

Gene Symbol

AGO2

Additional Ago2/eIF2C2 Products

Product Documents for Ago2/eIF2C2 Antibody (036) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ago2/eIF2C2 Antibody (036) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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