Recombinant Human AK3 His Protein

Novus Biologicals | Catalog # NBC1-18396

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all AK3 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1- 223 of Human AK3

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASK LLRAVILGPP GSGKGTVSQR IAQNFGLQHL SSGHFLRENI KASTEVGEMA KQYIEKSLLV PDHVITRLMM SELENRRGQH WLLDGFPRTL GQAEALDKIC EVDLVISLNI PFETLKDRLS RRWIHPPSGR VYNLDFNPPH VHGIDDVTGE PLVQQEDDKP EAVAARLRQY KDVAKPVIEL YKSRGVLHQF SGTETNKIWP YVYTLFSNKI TPIQSKEAY

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

29.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human AK3 His Protein

SDS-PAGE: Recombinant Human AK3 His Protein [NBC1-18396]

SDS-PAGE: Recombinant Human AK3 His Protein [NBC1-18396]

SDS-Page: AK3 Protein [NBC1-18396] - Adenylate kinase 3(C22S), 29.3 kDa (259 aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation, and Storage

NBC1-18396
Formulation 20 mM Tris-HCl buffer (pH 8.5), 2 mM DTT, 30% Glycerol, 0.1M NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AK3

Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Five isozymes of adenylate kinase have been identified in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell. Recombinant human AK3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

GTP:AMP phosphotransferase AK3, mitochondrial

Alternate Names

Adenylate kinase 3, AK 3, AK3L1, AK6, AKL3L, EC 2.7.4.10

Gene Symbol

AK3

Additional AK3 Products

Product Documents for Recombinant Human AK3 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human AK3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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